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Detection of melamine based on the suppressed anodic response of uric acid by a Au-Ag nanoparticles modified glassy carbon electrode

Yu-Hui Yu-Hui Peng, National Changhua University of Education, Tsunghsueh Tsunghsueh Wu, University of Wisconsin-Platteville, Yang-Wei Yang-Wei Lin, National Changhua University of Education
Journal of Food and Drug Analysis , Volume 28; doi:10.38212/2224-6614.1075

Abstract: We demonstrated a sensitive electrochemical method for the determination of non-electroactive melamine (Mel) using a modified glassy carbon electrode (GCE), with uric acid (UA) as the signal reporter. To increase the anodic response of UA, GCE was coated with Au–Ag nanoparticles and a Nafion thin film (Au–Ag/Nafion/GCE). The sensing mechanism was based on the competitive adsorption behavior of Mel on the Au–Ag/Nafion/GCE, which reduces the electroactive surface area of nanoparticles and thus hinders anodic response of UA. Under optimal conditions and the use of an analytical method of differential pulse voltammetry, this modified electrode detected Mel concentrations ranging from 2.5 to 70 nM, with a detection limit of 1.8 nM. The Au–Ag/Nafion/GCE demonstrated satisfactory reproducibility and stability, with relative standard deviations (RSDs) of 9.3% and 7.1%, respectively. The proposed electrochemical method was then successfully used to determine the Mel content in spiked milk powder and cat food samples, with RSDs of 1.7%–9.3% and recoveries of 92.4%–103.7%.
Keywords: Behavior / Differential / optimal / melamine / glassy / Rsds / Gce / Anodic Response

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