GC-MS/MS analysis of metabolites derived from a single human blastocyst
Open Access
- 25 January 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Metabolomics
- Vol. 17 (2), 1-7
- https://doi.org/10.1007/s11306-021-01770-x
Abstract
Introduction The field of assisted reproductive technology (ART) has significantly advanced; however, morphological evaluation remains as the chosen method of assessment of embryo quality. Objective We aimed to examine metabolic changes in embryo culture medium to develop a non-invasive method for evaluation of embryo quality. Methods We performed metabolic analysis of culture medium obtained from a single blastocyst cultured for freezing. Results In total, 187 (39.8%) of the 469 detectable organic acid metabolites were identified. A significant change (p < 0.05) was observed in eight metabolites between the good-quality and poor-quality embryo groups. Differences were observed in several metabolic pathways between the good-quality and poor-quality embryo groups. Metabolites that showed significant changes were primarily involved in the metabolism of branched-chain amino acids. Conclusion The quantification of metabolism in human embryos may assist in identification and selection of good-quality embryos with high rates of survival before freezing and implantation in conjunction with morphological classification. This may help to identify embryos with high rates of survival.This publication has 24 references indexed in Scilit:
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