Space syntax is one of the important methods for urban analysis and calculation. Based on the theory of space syntax, this study takes the Macau Conservatory of Music, an important Portuguese historical building, as an example, to first analyze the particularity of its development history in urban heritage, followed by the internal spatial structure, and import Depthmap for operation, combined with topology-related calculation methods. By analyzing the topological values such as the overall integration, local integration, global depth and selectivity of the space, we can quantitatively explore the structure of the space, understand the relationship between the overall space and the local space of the Macau Conservatory of Music, discover the deficiencies in the space structure, and propose corresponding optimization strategies are proposed to provide ideas and reference for the future renovation of the typical performing arts architectural space in the urban heritage of Macau.