Molecular Survey and Phylogenetic Analysis of Atypical Porcine Pestivirus (APPV) Identified in Swine and Wild Boar from Northern Italy
Open Access
- 10 December 2019
- Vol. 11 (12), 1142
- https://doi.org/10.3390/v11121142
Abstract
Atypical porcine pestivirus (APPV) is a newly recognized member of the Flaviviridae family. This novel porcine pestivirus was first described in 2015 in the USA, where it has been associated with congenital tremor type A-II in new-born piglets. APPV is widely distributed in domestic pigs in Europe and Asia. In this study, a virological survey was performed in Northern Italy to investigate the presence of APPV using molecular methods. Testing of 360 abortion samples from pig herds revealed two APPV strains from distinct provinces in the Lombardy region and testing of 430 wild boar blood samples revealed three strains, one from Lombardy and two from Emilia Romagna. The nucleotide sequencing of a fragment of the nonstructural protein 3-coding region revealed a high similarity to the previously detected European strains (Spanish, German, and Italian) of APPV.Keywords
This publication has 29 references indexed in Scilit:
- Presence of atypical porcine pestivirus (APPV) genomes in newborn piglets correlates with congenital tremorScientific Reports, 2016
- Identification of a Divergent Lineage Porcine Pestivirus in Nursing Piglets with Congenital Tremors and Reproduction of Disease following Experimental InoculationPLOS ONE, 2016
- Discovery of a novel putative atypical porcine pestivirus in pigs in the USAJournal of General Virology, 2015
- IQ-TREE: A Fast and Effective Stochastic Algorithm for Estimating Maximum-Likelihood PhylogeniesMolecular Biology and Evolution, 2014
- MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0Molecular Biology and Evolution, 2013
- Virome Analysis for Identification of Novel Mammalian Viruses in Bat Species from Chinese ProvincesJournal of Virology, 2012
- Phylogeny, classification and evolutionary insights into pestivirusesVirology, 2009
- Identification of a novel virus in pigs—Bungowannah virus: A possible new species of pestivirusVirus Research, 2007
- Optimized DNA microarray assay allows detection and genotyping of single PCR-amplifiable target copiesMolecular and Cellular Probes, 2006
- Comparison of porcine circovirus type 2 load in serum quantified by a real time PCR in postweaning multisystemic wasting syndrome and porcine dermatitis and nephropathy syndrome naturally affected pigsJournal of Virological Methods, 2004