Pharmacodynamics of colistin resistance in carbapenemase-producing Klebsiella pneumoniae: the double-edged sword of heteroresistance and adaptive resistance
Open Access
- 6 October 2022
- journal article
- research article
- Published by Microbiology Society in Journal of Medical Microbiology
- Vol. 71 (10), 001565
- https://doi.org/10.1099/jmm.0.001565
Abstract
Introduction. The presence of heteroresistant subpopulations and the development of resistance during drug exposure (adaptive resistance) limits colistin’s efficacy against carbapenemase-producing Klebsiella pneumoniae (CP-Kp) isolates. Hypothesis/Gap statement. The pharmacokinetic/pharmacodynamic (PK/PD) characteristics of both types of colistin resistance against CP-Kp are unknown. Aim. We therefore studied the PK/PD characteristics of colistin resistance in an in vitro PK/PD model simulating clinical colistin exposures. Methods. Two K. pneumoniae clinical isolates, one non-CP-Kp and one CP-Kp, with colistin MICs of 0.5–1 mg l−1 at a final inoculum of 107 c.f.u. ml−1 were used in an in vitro PK/PD dialysis/diffusion closed model simulating 4.5 MU q12h and 3 MU q8h clinical dosing regimens. Heteroresistant (HRS, bacteria with stable high-level resistance present before drug exposure) and adaptive resistant (ARS, bacteria with reversible low-level resistance emerging after drug exposure) subpopulations were measured and optimal PK/PD targets for reducing both ARS and HRS were determined. Cumulative fractional response (CFR) was calculated with Monte Carlo simulation for 9 MU q24h, 4.5 MU q12h and 3 MU q8h clinical dosing regimens. Results. A 2–5 log10c.f.u. ml–1 decrease of the total bacterial population was observed within the first 2 h of exposure, followed by regrowth at 12 h. Colistin exposure was positively and negatively correlated with HRS and ARS 24–0 h c.f.u. ml–1 changes, respectively. An optimal PK/PD (~0.5log10 increase) target of 35 fAUC/MIC (the ratio of the area under the unbound concentration–time curve to the MIC) was found for reducing both HRS and ARS of high-level resistance (MIC >16 mg l−1). The 4.5 MU q12h regimen had slightly higher CFR (74 %) compared to the other dosing regimens. Conclusions. High colistin exposures reduced high-level adaptive resistance at the expense of selection of heteroresistant subpopulations.Keywords
This publication has 22 references indexed in Scilit:
- Molecular mechanisms of polymyxin resistance: knowns and unknownsInternational Journal of Antimicrobial Agents, 2016
- Comparison of Short Versus Prolonged Infusion of Standard Dose of Meropenem Against Carbapenemase-Producing Klebsiella pneumoniae Isolates in Different Patient Groups: A Pharmacokinetic–Pharmacodynamic ApproachJournal of Pharmaceutical Sciences, 2016
- Mechanisms of polymyxin resistance: acquired and intrinsic resistance in bacteriaFrontiers in Microbiology, 2014
- KpnEF, a New Member of the Klebsiella pneumoniae Cell Envelope Stress Response Regulon, Is an SMR-Type Efflux Pump Involved in Broad-Spectrum Antimicrobial ResistanceAntimicrobial Agents and Chemotherapy, 2013
- Carbapenemases in Klebsiella pneumoniae and Other Enterobacteriaceae: an Evolving Crisis of Global DimensionsClinical Microbiology Reviews, 2012
- Application of a Loading Dose of Colistin Methanesulfonate in Critically Ill Patients: Population Pharmacokinetics, Protein Binding, and Prediction of Bacterial KillAntimicrobial Agents and Chemotherapy, 2012
- Pharmacodynamic Effects of Simulated Standard Doses of Antifungal Drugs against Aspergillus Species in a New In Vitro Pharmacokinetic/Pharmacodynamic ModelAntimicrobial Agents and Chemotherapy, 2012
- Multidrug-resistant Gram-negative bacteria: how to treat and for how longInternational Journal of Antimicrobial Agents, 2010
- Serum Bactericidal Activity of Three Different Dosing Regimens of Colistin with Implications for Optimum Clinical UseJournal of Chemotherapy, 2010
- Standardization of pharmacokinetic/pharmacodynamic (PK/PD) terminology for anti-infective drugs: an updateJournal of Antimicrobial Chemotherapy, 2005