Analysis of Biomechanical Parameters of Muscle Soleus Contraction and Blood Biochemical Parameters in Rat with Chronic Glyphosate Intoxication and Therapeutic Use of C60 Fullerene
Open Access
- 7 May 2021
- journal article
- research article
- Published by MDPI AG in International Journal of Molecular Sciences
- Vol. 22 (9), 4977
- https://doi.org/10.3390/ijms22094977
Abstract
The widespread use of glyphosate as a herbicide in agriculture can lead to the presence of its residues and metabolites in food for human consumption and thus pose a threat to human health. It has been found that glyphosate reduces energy metabolism in the brain, its amount increases in white muscle fibers. At the same time, the effect of chronic use of glyphosate on the dynamic properties of skeletal muscles remains practically unexplored. The selected biomechanical parameters (the integrated power of muscle contraction, the time of reaching the muscle contraction force its maximum value and the reduction of the force response by 50% and 25% of the initial values during stimulation) of muscle soleus contraction in rats, as well as blood biochemical parameters (the levels of creatinine, creatine phosphokinase, lactate, lactate dehydrogenase, thiobarbituric acid reactive substances, hydrogen peroxide, reduced glutathione and catalase) were analyzed after chronic glyphosate intoxication (oral administration at a dose of 10 μg/kg of animal weight) for 30 days. Water-soluble C60 fullerene, as a poweful antioxidant, was used as a therapeutic nanoagent throughout the entire period of intoxication with the above herbicide (oral administration at doses of 0.5 or 1 mg/kg). The data obtained show that the introduction of C60 fullerene at a dose of 0.5 mg/kg reduces the degree of pathological changes by 40–45%. Increasing the dose of C60 fullerene to 1 mg/kg increases the therapeutic effect by 55–65%, normalizing the studied biomechanical and biochemical parameters. Thus, C60 fullerenes can be effective nanotherapeutics in the treatment of glyphosate-based herbicide poisoning.This publication has 44 references indexed in Scilit:
- Structural Features of Highly Stable Reproducible C60Fullerene Aqueous Colloid Solution Probed by Various TechniquesFullerenes, Nanotubes and Carbon Nanostructures, 2014
- Interceptor effect of C60 fullerene on the in vitro action of aromatic drug moleculesEuropean Biophysics Journal, 2014
- Toxicological Responses of Cyprinus carpio Exposed to a Commercial Formulation Containing GlyphosateBulletin of Environmental Contamination and Toxicology, 2011
- Interaction of N-fluorescein-5-isothiocyanate pyrrolidine-C60 with a bimolecular lipid model membraneMaterials Science and Engineering: C, 2011
- Biochemical markers of muscular damagecclm, 2010
- Antibacterial Activity of Fullerene Water Suspensions: Effects of Preparation Method and Particle SizeEnvironmental Science & Technology, 2006
- [60]Fullerene is a Powerful Antioxidant in Vivo with No Acute or Subacute ToxicityNano Letters, 2005
- Effect of X-Ray and UV irradiation of the C60 fullerene aqueous solution on biological samplesCarbon, 2004
- Glyphosate PoisoningToxicological Reviews, 2004
- Radical Reactions of C 60Science, 1991