Mechanisms of DNA Uptake by Naturally Competent Bacteria
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- 3 December 2019
- journal article
- review article
- Published by Annual Reviews in Annual Review of Genetics
- Vol. 53 (1), 217-237
- https://doi.org/10.1146/annurev-genet-112618-043641
Abstract
Transformation is a widespread mechanism of horizontal gene transfer in bacteria. DNA uptake to the periplasmic compartment requires a DNA-uptake pilus and the DNA-binding protein ComEA. In the gram-negative bacteria, DNA is first pulled toward the outer membrane by retraction of the pilus and then taken up by binding to periplasmic ComEA, acting as a Brownian ratchet to prevent backward diffusion. A similar mechanism probably operates in the gram-positive bacteria as well, but these systems have been less well characterized. Transport, defined as movement of a single strand of transforming DNA to the cytosol, requires the channel protein ComEC. Although less is understood about this process, it may be driven by proton symport. In this review we also describe various phenomena that are coordinated with the expression of competence for transformation, such as fratricide, the kin-discriminatory killing of neighboring cells, and competence-mediated growth arrest. Expected final online publication date for the Annual Review of Genetics, Volume 53 is November 23, 2019. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.Keywords
This publication has 117 references indexed in Scilit:
- Specific DNA recognition mediated by a type IV pilinProceedings of the National Academy of Sciences of the United States of America, 2013
- Defining the DNA uptake specificity of naturally competent Haemophilus influenzae cellsNucleic Acids Research, 2012
- Competence in Streptococcus pneumoniae Is Regulated by the Rate of Ribosomal Decoding ErrorsmBio, 2011
- The Three-Layered DNA Uptake Machinery at the Cell Pole in Competent Bacillus subtilis Cells Is a Stable ComplexJournal of Bacteriology, 2011
- Antibiotics and UV Radiation Induce Competence for Natural Transformation inLegionella pneumophilaJournal of Bacteriology, 2011
- Membrane-associated DNA Transport MachinesCold Spring Harbor Perspectives in Biology, 2010
- Composite system mediates two-step DNA uptake into Helicobacter pyloriProceedings of the National Academy of Sciences of the United States of America, 2009
- The SOS Response Controls Integron RecombinationScience, 2009
- New Functional Identity for the DNA Uptake Sequence in Transformation and Its Presence in Transcriptional TerminatorsJournal of Bacteriology, 2007
- SOS response promotes horizontal dissemination of antibiotic resistance genesNature, 2003