Parameter Estimation in Different Enzyme Reactions
Open Access
- 1 January 2014
- journal article
- Published by Scientific Research Publishing, Inc. in Advances in Enzyme Research
- Vol. 02 (01), 14-26
- https://doi.org/10.4236/aer.2014.21002
Abstract
Enzyme kinetic parameters have been estimated using MATLAB software via the Wilkinson non-linear regression technique. The MATLAB script file written to implement this technique is short and very straightforward. Several software tools are commercially available for this purpose, with many graphical user interface (GUI) features. A routine use of these packages might offer immediate satisfaction of interactive hands-on experience; but in some cases the researcher might wish to write his/her own code and compare the results for further confirmation. Today MATLAB is in use in almost all the schools and laboratories as a standard software tool. So this paper is aimed at helping enzyme researchers to make use of this powerful software for estimation of parameters. It enables the incorporation of the analytical steps behind parameter estimation in an easy-to-follow manner and furnishes better visualization.Keywords
This publication has 12 references indexed in Scilit:
- SigrafW: An easy‐to‐use program for fitting enzyme kinetic dataBiochemistry and Molecular Biology Education, 2005
- On the convenience of using a computer simulation to teach enzyme kinetics to undergraduate students with biological chemistry‐related curriculaBiochemistry and Molecular Biology Education, 2003
- Optimization of kinetic parameters of enzymesBiochemistry and Molecular Biology Education, 2002
- On the error of the dixon plot for estimating the inhibition constant between enzyme and inhibitorBiochemistry and Molecular Biology Education, 2002
- An EXCEL template for calculation of enzyme kinetic parameters by non-linear regression.Bioinformatics, 1998
- ENZPACK: A Microcomputer Program to Aid in the Teaching of Enzyme KineticsBiochemical Education, 1983
- A Simple Digital‐Computer Program for Estimating the Parameters of the Hill EquationJBIC Journal of Biological Inorganic Chemistry, 1973
- Statistical estimations in enzyme kineticsBiochemical Journal, 1961
- A generalized inverse for matricesMathematical Proceedings of the Cambridge Philosophical Society, 1955
- The Determination of Enzyme Dissociation ConstantsJournal of the American Chemical Society, 1934