Effect of Different Oxygen Tension on Culture and Growth Pattern of Horse Adipose Tissue and Umbilical Cord Blood Derived Mesenchymal Stem Cells (hrs-AT-MSC and hrs-UCB-MSC)

Abstract

Equine multipotent mesenchymal stem cells (hrs-MSC) can be isolated from various tissues including Adipose Tissue (AT) and Umbilical Cord Blood (UCB). We have analyzed the effect of different oxygen tension on hrs-AT-MSC and hrs-UCB-MSC cultured and assessed proliferation, morphology, viability and immunophenotype and plasticity. Both type of equine MSC were cultured separately in normoxic (21% O2) and hypoxic (5% O2) environment