Sequence modification on demand: search and replace tools for precise gene editing in plants
- 4 June 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Transgenic Research
- Vol. 30 (4), 353-379
- https://doi.org/10.1007/s11248-021-00253-y
Abstract
Until recently, our ability to generate allelic diversity in plants was limited to introduction of variants from domesticated and wild species by breeding via uncontrolled recombination or the use of chemical and physical mutagens—processes that are lengthy and costly or lack specificity, respectively. Gene editing provides a faster and more precise way to create new variation, although its application in plants has been dominated by the creation of short insertion and deletion mutations leading to loss of gene function, mostly due to the dependence of editing outcomes on DNA repair pathway choices intrinsic to higher eukaryotes. Other types of edits such as point mutations and precise and pre-designed targeted sequence insertions have rarely been implemented, despite providing means to modulate the expression of target genes or to engineer the function and stability of their protein products. Several advancements have been developed in recent years to facilitate custom editing by regulation of repair pathway choices or by taking advantage of alternative types of DNA repair. We have seen the advent of novel gene editing tools that are independent of DNA double-strand break repair, and methods completely independent of host DNA repair processes are being increasingly explored. With the aim to provide a comprehensive review of the state-of-the-art methodology for allele replacement in plants, I discuss the adoption of these improvements for plant genome engineering.This publication has 230 references indexed in Scilit:
- Transcription Activator-Like Effector Nucleases Enable Efficient Plant Genome EngineeringPlant Physiology, 2012
- Suppression of Ku70/80 or Lig4 leads to decreased stable transformation and enhanced homologous recombination in riceNew Phytologist, 2012
- phiC31 Integrase-Mediated Site-Specific Recombination in BarleyPLOS ONE, 2012
- A Programmable Dual-RNA–Guided DNA Endonuclease in Adaptive Bacterial ImmunityScience, 2012
- In planta gene targetingProceedings of the National Academy of Sciences of the United States of America, 2012
- Genome editing with engineered zinc finger nucleasesNature Reviews Genetics, 2010
- OsKu70 Is Associated with Developmental Growth and Genome Stability in RicePlant Physiology, 2009
- The STRUCTURAL MAINTENANCE OF CHROMOSOMES 5/6 Complex Promotes Sister Chromatid Alignment and Homologous Recombination after DNA Damage inArabidopsis thalianaTHE PLANT CELL ONLINE, 2009
- Stable Recombinase-Mediated Cassette Exchange in Arabidopsis UsingAgrobacterium tumefaciensPlant Physiology, 2007
- Capture of genomic and T-DNA sequences during double-strand break repair in somatic plant cellsThe EMBO Journal, 1998