Association of SNP g.232 G>T Calpain Gene with Growth and Live Meat Quality Prediction using Ultrasound Images in Bali Cattle
Open Access
- 29 June 2021
- journal article
- research article
- Published by Indonesian Center for Animal Research and Development (ICARD) in Jurnal Ilmu Ternak dan Veteriner
- Vol. 26 (2), 49-56
- https://doi.org/10.14334/jitv.v26i2.2701
Abstract
Bali cattle (Bos javanicus) are native Indonesian cattle, domesticated from banteng (Bibos banteng). Genes that have an important role in meat quality are calcium-activated neutral protease genes, known as calpains (CAPN). The objective of this study was to evaluate the polymophisms of calpain gene SNP g.232 G>T by PCR-RFLP technique and its influence on growth trait and meat quality of Bali cattle detected by ultrasound imaging of longissimus dorsi thickness (LDT), back fat thickness (BFT), marbling score (MS), and intramuscular fat percentage (PIMF). The polymorphisms of CAPN1 gene were analyzed by PCR-RFLP using BglII restriction enzyme (n=52 cattle). The ultrasound images of longissimus dorsi muscle were carried out transversally and longitudinal between 12th -13th thoracic vertebrae then analyzed by Image-J NIH software. Result showed that SNP g.232 G>T of CAPN1 gene was polymorphic in Bali cattle. SNP g.232 G>T of CAPN1 gene in Bali cattle has higher diversity which was showed of 0.48 heterozygosity value and was in Hardy-Weinberg equilibrium. The polymorphisms of SNP g.232 G>T was associated significantly (P<0.05) with bodyweight at 730 days, marbling score (MS), and intramuscular fat percentage (PIMF). It suggests that the CAPN1 gene in Bali cattle is a candidate for Marker Assisted Selection (MAS), which influences body weight at 730 days, marbling score, and percentage of intramuscular fat.Keywords
This publication has 30 references indexed in Scilit:
- Effects of genetic variants for the bovine calpain gene on meat tendernessMolecular Biology Reports, 2014
- Factors Affecting Ultrasound Intramuscular Fat Content in Musculus Longissimus Dorsi of Beef Bulls Estimated with Bia Pro Plus Software / Czynniki wpływające na ultrasonograficzną ocenę udziału tłuszczu śródmięśniowego mięśnia najdłuższego grzbietu buhajów mięsnychAnnals of Animal Science, 2013
- A New Insight into the Role of Calpains in Post-mortem Meat Tenderization in Domestic Animals: A reviewAsian-Australasian Journal of Animal Sciences, 2013
- Before It Gets Started: Regulating Translation at the 5′ UTRComparative and Functional Genomics, 2012
- Indigenous Bali Cattle is Most Suitable for Sustainable Small Farming in IndonesiaReproduction in Domestic Animals, 2011
- Effects of polymorphisms in the 3′ untranslated region of the porcine PPARGC1A gene on muscle fiber characteristics and meat quality traitsMolecular Biology Reports, 2011
- Association between molecular markers for beef tenderness and growth traits in Argentinian Angus cattleAnimal Genetics, 2011
- Association between IGF-IR, m-calpain and UCP-3 gene polymorphisms and growth traits in Nanyang cattleMolecular Biology Reports, 2010
- A new SNP in the 3′UTR region of the bovine calpain small subunit (CAPNS1) geneMolecular Biology Reports, 2009
- A single nucleotide polymorphism in CAPN1 associated with marbling score in Korean cattleBMC Genomic Data, 2008