The detection of chymotrypsin using peptides covalent bound to the surface of graphene oxide and gold nanoparticles

Abstract

In this study, pyrene was used as a fluorescent dye to peptide. A sensitive, selective and simple sensor platform was built for detecting chymotrypsin based on the covalent binding peptide of gold nanoparticles (AuNPs) and GO. NaBr as a robust back-filler on gold for ensuring that the peptides were attached on the surface of Au NPs. Chymotrypsin was added to cleave the peptide specific site. The fluorescence was restored after the fluorescent group was released. The detection limit was 7.394 pg/mL. And the method can be applied in a real sample.