Insulin-like growth factor-1 gene expression in the endometrium of repeat breeder cows after the administration of presynch-10 and ovsynch protocol
- 3 March 2020
- journal article
- research article
- Published by Veterinary Journal of Ankara University in Ankara Üniversitesi Veteriner Fakültesi Dergisi
- Vol. 67 (2), 193-199
- https://doi.org/10.33988/auvfd.640288
Abstract
The aim of this study was to investigate the effects of using presynch-10 and ovsynch synchronization protocols on endometrial expression and serum levels of insulin-like growth factor-1 (IGF-1) that thought to play roles during endometrial healing process in cows diagnosed with repeat breeder syndrome (RBS) and subclinical endometritis (SE). A total of 40 Holstein-Friesian cows identified as having RBS and SE were used. The animals in Group 1 (G1, n=20) were applied presynch-10 and ovsynch furthermore, biopsy and blood samples were collected both before and after administration. Biopsy and blood samples were taken from the animals in Group 2 (G2, n=20) without performing any synchronization. The IGF-1 gene expression profiles were determined in all samples using RT-qPCR. Even though the level of IGF-1 expression increased in samples taken after synchronization in G1, it was not statistically significant (P>0.05). There was no statistical difference in serum IGF-1 levels between G1 and G2 (P>0.05). In conclusion, the expression of the IGF-1 gene in the endometrial tissue of RBS cows with SE was identified, but the evidence suggests that the synchronization protocols in question had no significant effect on the gene expression profile being investigated.Keywords
This publication has 33 references indexed in Scilit:
- Influence of energy balance on the somatotrophic axis and matrix metalloproteinase expression in the endometrium of the postpartum dairy cowReproduction, 2011
- Prevalence, risk factors for and impact of subclinical endometritis in repeat breeder dairy cowsTheriogenology, 2010
- Endometrial expression of the insulin-like growth factor system during uterine involution in the postpartum dairy cowDomestic Animal Endocrinology, 2008
- Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT MethodMethods, 2001
- The expression of the IGF system in the bovine uterus throughout the oestrous cycle and early pregnancyJournal of Endocrinology, 2000
- Expression of Growth Factor Ligand and Their Receptor mRNAs in Bovine Ova during In Vitro Maturation and after Fertilization In Vitro.The Journal of Veterinary Medical Science, 1998
- Synchronization of ovulation in dairy cows using PGF2α and GnRHTheriogenology, 1995
- The ovarian insulin and insulin-like growth factor system with an emphasis on domestic animalsDomestic Animal Endocrinology, 1995
- Expression of Messenger RNAs Encoding Insulin-like Growth Factor-I, -II, and Insulin-like Growth Factor Binding Protein-2 in Bovine Endometrium during the Estrous Cycle and Early Pregnancy1Biology of Reproduction, 1991
- Sequential endocrine changes and behaviour during oestrus and metoestrus in repeat breeder and virgin heifersAnimal Reproduction Science, 1986