Ultrasound reverses chemoresistance in breast cancer stem cell like cells by altering ABCG2 expression
Open Access
- 9 November 2017
- journal article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 37 (6)
- https://doi.org/10.1042/bsr20171137
Abstract
Doxorubicin (DOX) resistance in breast cancer largely results from the breast cancer stem cell like cells (BCSCs) which could be targetted to improve the efficacy of chemotherapy. Cell permeabilization using microbubbles (MBs) and ultrasound (US) have the potential for delivering molecules into the cytoplasm. We aim to evaluate a new methodology of US on BCSCs. First, our findings indicated that ALDHA1+ spheres which were derived from fresh primary breast cancer samples displayed stem cell like features and were resistant to DOX. In patient cohort, we revealed the presence of a variable fraction of ALDHA1+cells in nine out of ten. We, for the first time, showed a new US-MB treatment condition which could be used on ALDHA1+ BCSCs by fluorescence measurement and calcein assay. Next, we demonstrated the efficacy of combined treatment on human BCSCs in vitro and in vivo using DOX and US-MB: the combined treatment with much reduced drug dosage significantly suppressed the stem cell like features of BCSCs and induced BCSCs apoptosis. Furthermore, we suggested that decreased ABCG2 level might be one of the mechanisms by which US-MB medicated DOX treatment. In conclusion, this new US-MB treatment condition has clinical potential in breast cancer therapy by targetting BCSCs; thereby holding benefits for breast cancer patients.This publication has 15 references indexed in Scilit:
- The effects and mechanisms of SLC34A2 on maintaining stem cell-like phenotypes in CD147+breast cancer stem cellsTumor Biology, 2017
- CD44 is functionally crucial for driving lung cancer stem cells metastasis through Wnt/β‐catenin‐FoxM1‐Twist signalingMolecular Carcinogenesis, 2015
- Ultrasound elastography and contrast-enhanced ultrasound in infants, children and adolescentsEuropean Journal of Radiology, 2014
- Breast cancer statistics, 2013CA: A Cancer Journal for Clinicians, 2013
- Highly tumorigenic lung cancer CD133 + cells display stem-like features and are spared by cisplatin treatmentProceedings of the National Academy of Sciences of the United States of America, 2009
- Doxorubicin: The Good, the Bad and the Ugly EffectCurrent Medicinal Chemistry, 2009
- Identification and expansion of human colon-cancer-initiating cellsNature, 2006
- Mechanism of intracellular delivery by acoustic cavitationJapanese Journal of Clinical Oncology, 2006
- Local Delivery of Plasmid DNA Into Rat Carotid Artery Using UltrasoundJournal of the American College of Cardiology, 2002
- A cell initiating human acute myeloid leukaemia after transplantation into SCID miceNature, 1994