CXCL9 correlates with antitumor immunity and is predictive of a favorable prognosis in uterine corpus endometrial carcinoma
Open Access
- 8 February 2023
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Oncology
- Vol. 13, 1077780
- https://doi.org/10.3389/fonc.2023.1077780
Abstract
The C-X-C motif chemokine ligand-9 (CXCL9) is related to the progression of multiple neoplasms. Yet, its biological functions in uterine corpus endometrioid carcinoma (UCEC) remain shrouded in confusion. Here, we assessed the prognostic significance and potential mechanism of CXCL9 in UCEC. Firstly, bioinformatics analysis of the public cancer database, including the Cancer Genome Atlas / the Genotype-Tissue Expression project (TCGA+ GTEx, n=552) and Gene Expression Omnibus (GEO): GSE63678 (n=7), were utilized for the CXCL9 expression-related analysis in UCEC. It suggested that CXCL9 expression was significantly upregulated in UCEC patients. Then, the survival analysis of TCGA-UCEC was performed to reveal that hyper-expression of CXCL9 was related to prolonged survival. Then, the GSEA enrichment analysis showed various immune response-related pathways, including T/NK cell, lymphocyte activation, cytokine-cytokine receptor interaction network, and chemokine signaling pathway, mediated by CXCL9. Impressively, the cytotoxic molecules (IFNG, SLAMF7, JCHAIN, NKG7, GBP5, LYZ, GZMA, GZMB, and TNF3F9) and the immunosuppressive genes, including PD-L1, were positively related to the expression of CXCL9. Further, the immunohistochemistry assay of our validation cohort (n=124) showed that the CXCL9 protein expression was mainly located in intertumoral and significantly upregulated in the UCEC patients; UCEC with high intertumoral CXCL9 cell abundance harbored an improved prognosis; a higher ratio of anti-tumor immune cells (CD4+, CD8+, and CD56+ cell) and PD-L1 was found in UCEC with CXCL9 high expression. Consequently, we identified CXCL9 as an independent prognostic biomarker or therapeutic target in UCEC patients, which augmented anti-tumor immune effects to furnish survival benefits.Funding Information
- National Natural Science Foundation of China
This publication has 52 references indexed in Scilit:
- An alternatively spliced variant of CXCR3 mediates the metastasis of CD133+ liver cancer cells induced by CXCL9Oncotarget, 2016
- Immune evasion in cancer: Mechanistic basis and therapeutic strategiesSeminars in Cancer Biology, 2015
- Elevated serum levels of IL-2R, IL-1RA, and CXCL9 are associated with a poor prognosis in follicular lymphomaBlood, 2015
- PD-1 blockade induces responses by inhibiting adaptive immune resistanceNature, 2014
- Classification of endometrial carcinoma: more than two typesThe Lancet Oncology, 2014
- Immune cells: plastic players along colorectal cancer progressionJournal of Cellular and Molecular Medicine, 2013
- A myriad of functions and complex regulation of the CCR7/CCL19/CCL21 chemokine axis in the adaptive immune systemCytokine & Growth Factor Reviews, 2013
- CXCL9 induces chemotaxis, chemorepulsion and endothelial barrier disruption through CXCR3-mediated activation of melanoma cellsBritish Journal of Cancer, 2010
- Prostaglandin F2α-F-Prostanoid Receptor Signaling Promotes Neutrophil Chemotaxis via Chemokine (C-X-C Motif) Ligand 1 in Endometrial AdenocarcinomaCancer Research, 2009
- Combination of MIG (CXCL9) chemokine gene therapy with low-dose cisplatin improves therapeutic efficacy against murine carcinomaGene Therapy, 2006