KARRAGENANLAR VE ET PROTEİNLERİ ARASINDAKİ ETKİLEŞİMLERİN DİFFERANSİYEL TARAMALI KALORİMETRE İLE İNCELENMESİ
Open Access
- 1 October 2018
- journal article
- Published by Association of Food Technology, Turkey in GIDA / The Journal of Food
- Vol. 43 (5), 776-786
- https://doi.org/10.15237/gida.gd18061
Abstract
Tr en Bu çalışma kapsamında et proteinlerinin farklı karragenanlar ile etkileşimi NaCl eklendiği ve eklenmediği durumlarda araştırılmıştır. Bu amaçla et proteinlerinin denatürasyon pik sıcaklıkları (Tp) ve camsı geçiş sıcaklıkları (Tg) ve karragenanların jelleşme sıcaklıkları differansiyel taramalı kalorimetre (DTK) ile incelenmiştir. DTK’dan elde edilen termogramlarda miyosin için 57.96 ° C, sarkoplazmik proteinler için 66.08 ° C ve aktin için 79.23 ° C olmak üzere 3 temel Tp değeri bulunmuştur. Kıyma haline getirilmiş dana eti tuz eklendiğinde miyosin ve aktinin Tp değerlerinde azalma sarkoplazmik proteinlerininkinde ise artış gözlenmiştir. Karragenanlar eklendiğinde ise proteinlerin aktin ve miyosinin Tp değerlerinde azalma gözlenmiştir. Karragenanlar etkilerini tuz varlığında daha belirgin olarak göstermişlerdir (P ≤0.05). Camsı geçiş sıcaklığı DTK termogramlarında basamak değişim olarak gözlenmiştir. Tuz eklemenin camsı geçiş sıcaklığı üzerine önemli bir etkisi gözlenmezken (P >0.05), eklenen karragenanlardan yalnızca ι-karragenan artışa neden olmuştur (P ≤0.05). κ ve λ-karragenan ise camsı geçiş sıcaklıklarında azalmaya neden olmuşlardır (P >0.05). In this study the interactions between meat proteins and κ, ι, λ-carrageenans with or without NaCl were investigated. For this purpose the thermal denaturation (Tp) and glass transition temperatures (Tg) and gelation temperatures of carrageenans were determined by using differential scanning calorimetry (DSC). Three denaturation peaks were found for ground meat which were 57.96 ° C for myosin, 66.08 ° C for sarcoplasmic proteins and 79.23 ° C for actin. The Tp of myosin and actin were decreased while Tp of sarcoplasmic proteins were increased when NaCl was added. Addition of carrageenans decreased the Tp of actin and myosin and it was observed that effect of carrageenans was increased when salt was added. The Tg was observed as step change in the thermograms. Addition of salt did not affect Tg of samples, only ι-carrageenan increased Tg of samples (P ≤0.05). On the other hand κ, λ-carrageenan caused a decrease in the Tg of samples (P >0.05).Keywords
This publication has 15 references indexed in Scilit:
- EFFECT OF THE INTERACTION BETWEEN CARRAGEENAN, GELLAN GUM AND FLAXSEED GUM ON QUALITY ATTRIBUTES OF STARCH‐FREE EMULSION‐TYPE SAUSAGEJournal of Muscle Foods, 2010
- Comparison of the thermal characteristics of connective tissue in loose structured and normal structured porcine M. semimembranosusMeat Science, 2008
- Determination of glass transition temperature of beef and effects of various cryoprotective agents on some chemical changesMeat Science, 2008
- Effect of salt addition on the thermal behavior of proteins of bovine meat from ArgentinaMeat Science, 2008
- Effect of Temperature and Glassy States on the Molecular Mobility of Solutes in Frozen Tuna Muscle As Studied by Electron Spin Resonance Spectroscopy with Spin Probe DetectionJournal of Agricultural and Food Chemistry, 2004
- Hydrocolloids at interfaces and the influence on the properties of dispersed systemsFood Hydrocolloids, 2003
- Effects of carrageenan on thermal stability of proteins from chicken thigh and breast musclesFood Research International, 2001
- Carrageenan Effects on Thermal Stability of Meat ProteinsJournal of Food Science, 1997
- Differential Scanning Calorimetry of Beef/Kappa‐Carrageenan MixturesJournal of Food Science, 1994
- Differential scanning calorimetric studies of muscle and its constituent proteinsJournal of the Science of Food and Agriculture, 1977