DETERMINATION OF MICROBIOLOGICAL QUALITY AND PRESENCE OF SALMONELLA SPP. ON CHICKEN PARTS SOLD AT RETAIL MARKETS IN ERZURUM, ANTIBIOTIC RESISTANCE OF THE SALMONELLA SPP. ISOLATES
Open Access
- 1 August 2019
- journal article
- Published by Association of Food Technology, Turkey in GIDA / The Journal of Food
- Vol. 44 (4), 553-562
- https://doi.org/10.15237/gida.gd18086
Abstract
En tr The present study was conducted to investigate the certain microbiological quality characteristics, and presence of Salmonella spp. by immunomagnetic separation (IMS) and conventional method, also to evaluate the antibiotic resistance of Salmonella spp. isolates obtained from 45 chicken meat samples (fifteen livers, chests, baguettes) collected from markets at the Erzurum. Totally, in the 15 samples were found positive for Salmonella spp. by IMS and conventional methods. Isolates were identified with 99.9% probability using API 20E biochemical analysis kit. The results of antibiotic test showed the susceptibility of the isolates to ciprofloxacin, chloramphenicol and gentamicin at 100% level, while these isolates were resistance to tetracycline, trimethoprim, sulfamethoxazole/trimethoprim, nalidixic acid, streptomycin, ampicillin and kanamycin, respectively. The obtained results showed that 84% (38/45) of the chicken meat samples did not show suitability with Turkish Food Codex in terms of total bacteria count and presence of Salmonella in 33% (15/45) of the samples. Bu çalışma, Erzurum’da marketlerden toplanan 45 adet tavuk eti örneğinde (15’er ciğer, göğüs, baget) belirli mikrobiyolojik kalite karakteristikleri, immunomanyetik separasyon (IMS) ve geleneksel yöntem ile Salmonella spp. varlığını araştırmak için yapılmıştır. Ayrıca, Salmonella spp. izolatlarının antibiyotik dirençleri değerlendirilmiştir. Toplamda 15 örnekte IMS ve geleneksel metot ile Salmonella spp. pozitif bulunmuştur. Bu izolatlar API 20E biyokimyasal analiz kiti kullanılarak %99,9 ihtimalle doğrulanmıştır. Salmonella spp. izolatları siprofloksasin, kloromfenikol ve gentamisin antibiyotiklerine karşı %100 seviyesinde duyarlılık gösterirken, sırasıyla tetrasiklin, trimetoprim, sülfametoksazol/trimethoprim, nalidiksik asit, streptomisin, ampisilin ve kanamisin antibiyotiklerine karşı direnç göstermiştir. Elde edilen sonuçlara göre tavuk etlerinin %84’ü (38/45) toplam bakteri sayısı, %33’ünün (15/45) Salmonella spp. varlığı açısından Türk Gıda Kodeks’ine uygun olmadığı görülmüştür.Keywords
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