Heat-stress-induced sprouting and differential gene expression in growing potato tubers: Comparative transcriptomics with that induced by postharvest sprouting
Open Access
- 15 October 2021
- journal article
- research article
- Published by Oxford University Press (OUP) in Horticulture Research
- Vol. 8 (1), 1-18
- https://doi.org/10.1038/s41438-021-00680-2
Abstract
Crops face increased risk from heat stress due to climate change. Potato (Solanum tuberosum L.) tubers grown in hot summers often have defects including pre-harvest sprouting (“heat sprouts”). We have used 18 potato cultivars to investigate whether heat stress (HS) conditions alone could cause heat sprouting and dormancy changes in tubers. We also examined transcriptomic responses of potato to HS and whether these responses are like those induced by postharvest sprouting. We demonstrated that HS alone caused heat sprouts and shortened postharvest dormancy period, heat-sprouted tubers became dormant after harvest, and cultivars varied substantially for producing heat spouts but there was no clear association with cultivar maturity earliness. Cultivar Innovator did not show any heat sprouts and still had long dormancy. Dormancy-associated genes (DOG1 and SLP) were downregulated in HS tubers like in postharvest sprouting tubers. We have identified 1201 differentially expressed genes, 14 enriched GO terms and 12 enriched KEGG pathways in response to HS in growing tubers of ‘Russet Burbank’. Transcriptomic response of ‘Russet Burbank’ to HS showed significant similarities to that of postharvest non-HS sprouted tubers. Gibberellin biosynthesis pathway was enriched in heat-stressed tubers and was likely involved in heat sprouting and dormancy release. Heat sprouting and postharvest sprouting shared common candidate genes and had significant similarity in gene expression. Our study has significance for selecting potato cultivars for farming, planning storage and utilization of heat-stressed tubers, identifying sprouting-related genes, understanding heat-stress biology, and breeding heat-tolerant potato cultivars, especially for sustainable potato production under climate change.Keywords
Funding Information
- Agriculture and Agri-Food Canada-Food Canada A-base research funding
- National Natural Science Foundation of China (31560413)
- Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada (RGPIN 2017-04589)
This publication has 45 references indexed in Scilit:
- KOBAS 2.0: a web server for annotation and identification of enriched pathways and diseasesNucleic Acids Research, 2011
- Systematic analysis of potato acid invertase genes reveals that a cold-responsive member, StvacINV1, regulates cold-induced sweetening of tubersMolecular Genetics and Genomics, 2011
- Differential expression analysis for sequence count dataGenome Biology, 2010
- Molecular characterization and biotechnological improvement of the processing quality of potatoesCanadian Journal of Plant Science, 2008
- Adaptation of potato to high temperatures and salinity-a reviewAmerican Journal of Potato Research, 2007
- CYP707A1 and CYP707A2, Which Encode Abscisic Acid 8′-Hydroxylases, Are Indispensable for Proper Control of Seed Dormancy and Germination in ArabidopsisPlant Physiology, 2006
- Evolution, structure and function of the small heat shock proteins in plantsJournal of Experimental Botany, 1996
- Impact of early-season water deficits on Russet Burbank plant development, tuber yield and qualityAmerican Journal of Potato Research, 1992
- Potato hair sprout disorder of potatoes causes problems for processors and seed producersAmerican Journal of Potato Research, 1956
- Studies on the association of heat sprouting with rest period and maturing time in Irish potatoesAmerican Journal of Potato Research, 1943