Characterization of fungal microbial diversity in healthy and diarrheal Tibetan piglets
Open Access
- 3 July 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in BMC Microbiology
- Vol. 21 (1), 1-10
- https://doi.org/10.1186/s12866-021-02242-x
Abstract
Background: Diarrhea is an important ailment limiting the production of the Tibetan pig industry. Dynamic balance of the intestinal microbiota is important for the physiology of the animal. The objective of this work was to study fungal diversity in the feces of early weaning Tibetan piglets in different health conditions. Results: In the present study, we performed high-throughput sequencing to characterize the fungal microbial diversity in healthy, diarrheal and treated Tibetan piglets at the Tibet Autonomous Region of the People’s Republic of China. The four alpha diversity indices (Chao1, ACE, Shannon and Simpson) revealed no significant differences in the richness across the different groups (P > 0.05). In all samples, the predominant fungal phyla were Ascomycota, Basidiomycota and Rozellomycota. Moreover, the healthy piglets showed a higher abundance of Ascomycota than the treated ones with a decreased level of Basidiomycota. One phylum (Rozellomycota) showed higher abundance in the diarrheal piglets than in the treated. At genus level, compared with that to the healthy group, the proportion of Derxomyces and Lecanicillium decreased, whereas that of Cortinarius and Kazachstania increased in the diarrheal group. The relative abundances of Derxomyces, Phyllozyma and Hydnum were higher in treated piglets than in the diarrheal ones. Conclusions: A decreased relative abundance of beneficial fungi (e.g. Derxomyces and Lecanicillium) may cause diarrhea in the early-weaned Tibetan piglets. Addition of probiotics into the feed may prevent diarrhea at this stage. This study presented the fungal diversity in healthy, diarrheal and treated early-weaned Tibetan piglets.Keywords
Funding Information
- Tibet Autonomous Region Department and College Joint Foundation-Key Project (XZ2019ZRG-55(Z), XZ2019ZRG-55(Z), XZ2019ZRG-55(Z))
- Tibet Autonomous Region Science and Technology Plan Project-Key Research and Development Program (XZ202001ZY0039N, XZ202001ZY0039N, XZ202001ZY0039N, XZ202001ZY0039N)
- Central Government Supports Local Colleges and Universities to Develop Special Funds Projects (ZZXT2019-02, ZZXT2019-02, ZZXT2019-02, ZZXT2019-02, ZZXT2019-02)
This publication has 42 references indexed in Scilit:
- Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencingNature Methods, 2013
- Microbial shifts in the swine distal gut in response to the treatment with antimicrobial growth promoter, tylosinProceedings of the National Academy of Sciences of the United States of America, 2012
- A Proposed Taxonomy of Anaerobic Fungi (Class Neocallimastigomycetes) Suitable for Large-Scale Sequence-Based Community Structure AnalysisPLOS ONE, 2012
- Human gut microbiome viewed across age and geographyNature, 2012
- In-feed antibiotic effects on the swine intestinal microbiomeProceedings of the National Academy of Sciences of the United States of America, 2012
- Novel strategies and approaches to develop the next generation of vaccines against porcine reproductive and respiratory syndrome virus (PRRSV)Virus Research, 2010
- QIIME allows analysis of high-throughput community sequencing dataNature Methods, 2010
- Statistical Methods for Detecting Differentially Abundant Features in Clinical Metagenomic SamplesPLoS Computational Biology, 2009
- Species Diversity and Utilization of Medicinal Mushrooms and Fungi in China (Review)International Journal of Medicinal Mushrooms, 2009
- Multivariate analyses in microbial ecologyFEMS Microbiology Ecology, 2007