Morphologic study of the laryngeal taste buds in the cat
- 1 December 1995
- journal article
- Published by Wiley in The Laryngoscope
- Vol. 105 (12), 1315-1321
- https://doi.org/10.1288/00005537-199512000-00010
Abstract
The distribution of laryngeal taste buds (TBs) and their neutral components in the cat were investigated by immunohistochemistry and electron microscopy. The antisera used in this study were against cytokeratin, protein gene product 9.5 (PGP9.5), neuron-specific enolase (NSE), S-100 protein, calbindin D, calcitonin gene-related peptide (CGRP), and substance P (SP). Taste bud cells were specifically immunoreactive to the antibodies of human cytokeratin subtypes 8 and 18 (CAM5.2). On observation with CAM5.2, TBs were seen distributed on the laryngeal surface of the epiglottis and spread caudally along the aryepiglottic folds, reaching peak density at the laryngeal side of the arytenoid tubercle. The PGP9.5 and NSE immunoreactivities were recognized in TB cells and nerve fibers, both within the TBs and in the subepithelial connective tissue. S-100 protein immunoreactivities were not found in any of the cells in the TBs but were found exclusively in the subepithelial neural elements. The calbindin-D, CGRP, and SP immunoreactivities were confined to a part of the neural elements that was very thin. Taste pores, taste villi, neuronal varicosity, and synapselike structures were observed by scanning and transmission electron microscopic study. From these results it is considered that the TBs act as a chemical receptor.Keywords
This publication has 23 references indexed in Scilit:
- Calbindin-like immunoreactivity in two peripheral chemosensory tissues of the rat: taste buds and the vomeronasal organBrain Research, 1992
- Cytokeratin Polypeptide Profile of Merkel Cells in Human Fetal and Adult Skin: Difference of Expression of Cytokeratins in Epidermal and Dermal Merkel CellsJournal of Investigative Dermatology, 1992
- Taste bud distribution in the rat pharynx and larynxThe Anatomical Record, 1990
- Fine structure of the taste bud in guinea pigs. I. Cell characterization and innervation patterns.Archives of Histology and Cytology, 1990
- Immunocytochemistry of neuron-specific proteins and neuropeptides in taste buds and associated nerves.Archives of Histology and Cytology, 1989
- Immunocytochemical localizations of neuron-specific proteins in the taste bud of the guinea pig.Archives of Histology and Cytology, 1988
- Quantitative taste bud distribution in the hamsterPhysiology & Behavior, 1984
- The cytologic composition of primate laryngeal chemosensory corpusclesJournal of Anatomy, 1980
- Fine structure of gustatory cells in rabbit taste budsJournal of Ultrastructure Research, 1969
- Number and distribution of taste buds on the epiglottis, pharynx, larynx, soft palate and uvula in a human newbornThe Anatomical Record, 1961