A diagnostic marker kit for Fusarium wilt and sterility mosaic diseases resistance in pigeonpea
Open Access
- 20 October 2020
- journal article
- research article
- Published by Springer Science and Business Media LLC in Theoretical and Applied Genetics
- Vol. 134 (1), 367-379
- https://doi.org/10.1007/s00122-020-03702-0
Abstract
Fusarium wilt (FW) and sterility mosaic diseases (SMD) are key biotic constraints to pigeonpea production. Occurrence of these two diseases in congenial conditions is reported to cause complete yield loss in susceptible pigeonpea cultivars. Various studies to elucidate genomic architecture of the two traits have revealed significant marker–trait associations for use in breeding programs. However, these DNA markers could not be used effectively in genomics-assisted breeding for developing FW and SMD resistant varieties primarily due to pathogen variability, location or background specificity, lesser phenotypic variance explained by the reported QTL and cost-inefficiency of the genotyping assays. Therefore, in the present study, a novel approach has been used to develop a diagnostic kit for identification of suitable FW and SMD resistant lines. This kit was developed with 10 markers each for FW and SMD resistance. Investigation of the diversity of these loci has shown the role of different alleles in different resistant genotypes. Two genes (C.cajan_03691 and C.cajan_18888) for FW resistance and four genes (C.cajan_07858, C.cajan_20995, C.cajan_21801 and C.cajan_17341) for SMD resistance have been identified. More importantly, we developed a customized and cost-effective Kompetitive allele-specific PCR genotyping assay for the identified genes in order to encourage their downstream applications in pigeonpea breeding programs. The diagnostic marker kit developed here will offer great strength to pigeonpea varietal development program, since the resistance against these two diseases is essentially required for nominating an improved line in varietal release pipeline.Keywords
This publication has 58 references indexed in Scilit:
- Large-Scale Development of Cost-Effective Single-Nucleotide Polymorphism Marker Assays for Genetic Mapping in Pigeonpea and Comparative Mapping in LegumesDNA Research, 2012
- An intra-specific consensus genetic map of pigeonpea [Cajanus cajan (L.) Millspaugh] derived from six mapping populationsTheoretical and Applied Genetics, 2012
- Genetic Patterns of Domestication in Pigeonpea (Cajanus cajan (L.) Millsp.) and Wild Cajanus RelativesPLOS ONE, 2012
- Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource-poor farmersNature Biotechnology, 2011
- A framework for variation discovery and genotyping using next-generation DNA sequencing dataNature Genetics, 2011
- Pigeonpea composite collection and identification of germplasm for use in crop improvement programmesPlant Genetic Resources: Characterization and Utilization, 2011
- MYB transcription factors in ArabidopsisTrends in Plant Science, 2010
- Simple sequence repeat‐based diversity in elite pigeonpea genotypes for developing mapping populations to map resistance to Fusarium wilt and sterility mosaic diseasePlant Breeding, 2010
- The Puf family of RNA-binding proteins in plants: phylogeny, structural modeling, activity and subcellular localizationBMC Plant Biology, 2010
- Identification of two RAPD markers genetically linked to a recessive allele of a Fusarium wilt resistance gene in pigeonpea (Cajanus cajan L. Millsp.)Euphytica, 2006