Recent advances in the microbial synthesis of lactate-based copolymer
Open Access
- 22 October 2021
- journal article
- review article
- Published by Springer Science and Business Media LLC in Bioresources and Bioprocessing
- Vol. 8 (1), 1-17
- https://doi.org/10.1186/s40643-021-00458-3
Abstract
Due to the increasing environmental pollution of un-degradable plastics and the consumption of non-renewable resources, more attention has been attracted by new bio-degradable/based polymers produced from renewable resources. Polylactic acid (PLA) is one of the most representative bio-based materials, with obvious advantages and disadvantages, and has a wide range of applications in industry, medicine, and research. By copolymerizing to make up for its deficiencies, the obtained copolymers have more excellent properties. The development of a one-step microbial metabolism production process of the lactate (LA)-based copolymers overcomes the inherent shortcomings in the traditional chemical synthesis process. The most common lactate-based copolymer is poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)], within which the difference of LA monomer fraction will cause the change in the material properties. It is necessary to regulate LA monomer fraction by appropriate methods. Based on synthetic biology and systems metabolic engineering, this review mainly focus on how did the different production strategies (such as enzyme engineering, fermentation engineering, etc.) of P(LA-co-3HB) optimize the chassis cells to efficiently produce it. In addition, the metabolic engineering strategies of some other lactate-based copolymers are also introduced in this article. These studies would facilitate to expand the application fields of the corresponding materials.Funding Information
- The National Natural Science Foundation of China (21776083)
- National Key Research and Development Program of China (2017YFB0309302)
- Fundamental Research Funds for the Central Universities (22221818014)
- the 111 Project (B18022)
This publication has 82 references indexed in Scilit:
- Metabolic engineering of Ralstonia eutropha for the biosynthesis of 2-hydroxyacid-containing polyhydroxyalkanoatesMetabolic Engineering, 2013
- Biosynthetic polyesters consisting of 2-hydroxyalkanoic acids: current challenges and unresolved questionsApplied Microbiology and Biotechnology, 2013
- Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery CarrierPolymers, 2011
- Lactate fraction dependent mechanical properties of semitransparent poly(lactate-co-3-hydroxybutyrate)s produced by control of lactyl-CoA monomer fluxes in recombinant Escherichia coliJournal of Biotechnology, 2011
- Polylactic acid (PLA): Research, development and industrializationBiotechnology Journal, 2010
- Microbial Production of Lactate-Enriched Poly[(R)-lactate-co-(R)-3-hydroxybutyrate] with Novel Thermal PropertiesBiomacromolecules, 2009
- Chemo-Enzymatic Synthesis of Poly(lactate-co-(3-hydroxybutyrate)) by a Lactate-Polymerizing EnzymeMacromolecules, 2009
- A microbial factory for lactate-based polyesters using a lactate-polymerizing enzymeProceedings of the National Academy of Sciences of the United States of America, 2008
- Evolution of Polyhydroxyalkanoate (PHA) Production System by “Enzyme Evolution”: Successful Case Studies of Directed EvolutionMacromolecular Bioscience, 2004
- Properties of lactic acid based polymers and their correlation with compositionProgress in Polymer Science, 2002