Spot morphology of non-contact printed protein molecules on non-porous substrates with a range of hydrophobicities
- 1 January 2013
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 138 (2), 518-524
- https://doi.org/10.1039/c2an36104c
Abstract
Non-contact inkjet printing technology is one of the most promising tools for producing microarrays. The quality of the microarray depends on the type of the substrate used for printing biomolecules. Various porous and non-porous substrates have been used in the past, but due to low production cost and easy availability, non-porous substrates like glass and plastic are preferred over porous substrates. On these non-porous substrates, obtaining spot uniformity and a high signal to noise ratio is a big challenge. In our research work, we have modified pristine glass slides using various silanes to produce a range of hydrophobic glass substrates. The hydrophobicities of the slides expressed in the contact angle (θ) of a sessile drop of water were 49°, 61°, 75°, 88° and 103°. Using a non-contact inkjet printer, microarrays of biotinylated biomolecules (BSA and IgG) were produced on these modified glass substrates, pristine (untreated) glass and also on HTA polystyrene slides. The uniformity of the spots, reflecting the distribution of the biomolecules in the spots, was analyzed and compared using confocal laser scanning microscopy (CLSM). The quality of the spots was superior on the glass slide with a contact angle of 75°. We also investigated the influence of the hydrophobicity of the substrate on a two-step, real diagnostic antibody assay. This nucleic acid microarray immunoassay (NAMIA) for the detection of Staphylococcus aureus showed that on highly hydrophilic (θ < 10°) and hydrophobic substrates (θ > 100°) the assay signal was low, whereas an excellent signal was obtained on the substrates with intermediate contact angles, θ 61° and θ 75°, respectively.Keywords
This publication has 20 references indexed in Scilit:
- Carbon Nanoparticles as Detection Labels in Antibody Microarrays. Detection of Genes Encoding Virulence Factors in Shiga Toxin-Producing Escherichia coliAnalytical Chemistry, 2011
- Printed protein microarrays on unmodified plastic substratesAnalytica Chimica Acta, 2010
- “Print-n-Shrink” technology for the rapid production of microfluidic chips and protein microarraysLab on a Chip, 2009
- New 3-D microarray platform based on macroporous polymer monolithsAnalytica Chimica Acta, 2009
- High performance protein microarrays based on glycidyl methacrylate-modified polyethylene terephthalate plastic substrateTalanta, 2009
- Surface modification of thermoplastics—towards the plastic biochip for high throughput screening devicesLab on a Chip, 2007
- An Aptamer-Based Protein BiochipAnalytical Chemistry, 2005
- Real-time analysis of protein adsorption to a variety of thin filmsBiosensors and Bioelectronics, 2004
- Synergistic effects of epoxy- and amine-silanes on microarray DNA immobilization and hybridizationBiochemical Journal, 2003
- Protein chip technologyCurrent Opinion in Chemical Biology, 2003