Application of experiment planning in the development of a nutrient medium in the production cycle of cultivation of G.Mellonella

Abstract

Relevance. The study aimed to develop the composition of an artificial nutrient medium for the rearing of Gallery mellonella larvae. In the process of the research, the relative contributions of the feed component to the biological growth parameters of larvae and their morphological indicators were determined.Methods. In the experiment the plan 2^7-4 was used, that is, fractional factor experiments were varied in which seven factors — feed ingredients. The matrix of the complete factor experiment 23 was taken as a basis, and the coefficients in the interaction of three or more factors were assumed to be insignificant and replaced by additional factors. Morphophysiological parameters of larvae were used as the main indicators: mass, length, width of the head capsule.Results To obtain biomass in the production cycle, the mass of larvae should be at least 0.15 g, length 20-22 mm, width of the head capsule 1.9-2.3 mm (VI-early VII age), survival rate of at least 85%. In the regression equation for larval masses, wheat flour (X₁), yeast (X₃) and honey (X₅) turned out to be significant ingredients. In the regression equation for the length of the larvae, the ingredients that make the maximum contribution are again yeast (X₃) and honey (X₅). Yeast (X₃) was significant for the width of the head capsule. The obtained regression equations enable mathematical modeling within the framework of a linear model represented by regression equations in the studied range of morphological parameters of larvae, depending on the composition of the feed.