Therapeutic peptides of Mucuna pruriens L.: Anti‐genotoxic molecules against human hepatocellular carcinoma and hepatitis C virus
Open Access
- 25 March 2021
- journal article
- research article
- Published by Wiley in Food Science & Nutrition
- Vol. 9 (6), 2908-2914
- https://doi.org/10.1002/fsn3.2248
Abstract
To assist the development of new therapeutic strategies for several disorders, biologically active peptides/proteins obtained from plant sources can be considered. Current study expected to determine the biological activities of peptide fractions of Mucuna pruriens against hepatocellular carcinoma cell lines (HepG2/ADM, HepG2, SMMC‐7721, and QGY‐7703), as well as normal cell line to prove their selectivity. Moreover, anti‐genotoxicity and antiviral activity against the hepatitis C virus (HCV) were assessed. The methods of this study were to isolate the peptides of M. pruriens and hydrolysate fractionation via fractionated pepsin‐pancreatin hydrolysates by ultrafiltration/high‐performance ultrafiltration cell, identify anti‐hepatoma activity of peptide fractions human liver cancer and normal cells by (3‐(4,5‐dimethylthiazole‐2‐yl)‐2,5‐biphenyl tetrazolium bromide) (MTT) assay, determine anti‐HCV, and assess anti‐genotoxic effect of peptide fractions against damage that induced via alkylating agent methyl methanesulphonate in human mononuclear cells. The results showed that the fraction 5–10 kDa has been reported to exhibit significant cytotoxic activity against HepG2 and QGY‐7703. It was proven that both of 5–10 and >10 kDa fractions are active against HCV. The cytotoxic concentration (CC50) of 5–10 kDa against the cell line was 703.04 ± 5.21 µg/ml. Anti‐genotoxic activities of the peptide fractions were evaluated as mean values for the analyzed comet images. In this regard, the highest activity of protecting DNA damages was observed by the peptide fraction of 5–10 kDa. This study revealed the potential ability of peptide fractions of M. pruriens for the treatment of liver cancer, HCV, and high activities of protecting DNA damages.Keywords
Funding Information
- Ferdowsi University of Mashhad (45440)
This publication has 27 references indexed in Scilit:
- Cytotoxicity of different extracts of Mucuna pruriensseeds on hepatoma cells but not on normal hepatic cellsPharmacognosy Communications, 2014
- Foliar application of Ca(NO3)2 and KNO3 affects growth, essential oil content, and oil composition of French tarragonIndustrial Crops and Products, 2014
- Structural characterization of the HSP70 interaction domain of the hepatitis C viral protein NS5AVirology, 2014
- Biological activities of the antiviral protein BE27 from sugar beet (Beta vulgaris L.)Planta, 2014
- ACE inhibitory, hypotensive and antioxidant peptide fractions from Mucuna pruriens proteinsProcess Biochemistry, 2014
- Determination of allelopathic potential in some medicinal and wild plant species of Iran by dish pack methodTheoretical and Experimental Plant Physiology, 2014
- Bunium persicum: variability in essential oil and antioxidants activity of fruits from different Iranian wild populationsGenetic Resources and Crop Evolution, 2014
- Antigenotoxic Potential of Aqueous Extracts from the Chanterelle Mushroom, Cantharellus cibarius (Higher Basidiomycetes), on Human Mononuclear Cell CulturesInternational Journal of Medicinal Mushrooms, 2013
- EFFECT OF NITROGEN FERTILIZER ON VEGETATIVE AND REPRODUCTIVE GROWTH OF PEPPER PLANTS UNDER FIELD CONDITIONSJournal of Plant Nutrition, 2012
- Antigenotoxicity of peptides produced from silk fibroinProcess Biochemistry, 2002