Discovery of physalin biosynthesis and structure modification of physalins in Physalis alkekengi L. var. Franchetii
Open Access
- 10 October 2022
- journal article
- research article
- Published by Frontiers Media SA in Frontiers in Plant Science
- Vol. 13, 956083
- https://doi.org/10.3389/fpls.2022.956083
Abstract
Physalins, active ingredients from the Physalis alkekengi L. var. franchetii (P. alkekengi) plant, have shown anti-inflammatory, antioxidant and anticancer activities. Whereas the bioactivity of physalins have been confirmed, their biosynthetic pathways, and those of quite a few derivatives, remain unknown. In this paper, biosynthesis and structure modification-related genes of physalins were mined through transcriptomic and metabolomic profiling. Firstly, we rapidly and conveniently analyzed physalins by UPLC-Q-TOF-MS/MS utilizing mass accuracy, diagnostic fragment ions, and common neutral losses. In all, 58 different physalin metabolites were isolated from P. alkekengi calyxes and berries. In an analysis of the physalin biosynthesis pathway, we determined that withanolides and withaphysalins may represent a crucial intermediate between lanosterol and physalins. and those steps were decanted according to previous reports. Our results provide valuable information on the physalin metabolites and the candidate enzymes involved in the physalins biosynthesis pathways of P. alkekengi. In addition, we further analyzed differential metabolites collected from calyxes in the Jilin province (Daodi of P. alkekengi) and other provinces. Among them, 20 physalin metabolites may represent herb quality biomarkers for Daodi P. alkekengi, providing an essential role in directing the quality control index of P. alkekengi.Keywords
This publication has 30 references indexed in Scilit:
- Three new physalins from Physalis alkekengi var. franchetiiNatural Products and Bioprospecting, 2013
- Physalin F Induces Cell Apoptosis in Human Renal Carcinoma Cells by Targeting NF-kappaB and Generating Reactive Oxygen SpeciesPLOS ONE, 2012
- Composition, Diversity, and Origin of the Bacterial Community in Grass Carp IntestinePLOS ONE, 2012
- Withanolides and Related SteroidsPublished by Springer Science and Business Media LLC ,2011
- ‘Omics’ analyses of regulatory networks in plant abiotic stress responsesCurrent Opinion in Plant Biology, 2010
- Widely Targeted Metabolomics Based on Large-Scale MS/MS Data for Elucidating Metabolite Accumulation Patterns in PlantsPlant and Cell Physiology, 2008
- Towards the plant metabolome and beyondNature Reviews Molecular Cell Biology, 2007
- Arabidopsiscyp51Mutant Shows Postembryonic Seedling Lethality Associated with Lack of Membrane IntegrityPlant Physiology, 2005
- Transporters of secondary metabolitesCurrent Opinion in Plant Biology, 2005
- Metabolomics: Current analytical platforms and methodologiesTrAC Trends in Analytical Chemistry, 2005