Hydrochemical and sanitary microbiological research of the Elena Hill spring waters in the Nakhunao community of Martvili Municipality was done for the first time.The content of Mg2+, Ca2+, Fe3+, HCO3-, Cl-, I- ions was determined. Relatively simple and quick chemical and physico-chemical methods of good repeatability were selected for determination. Biogenic substances were determined by photometric method.Carbon dioxide, oxygen BOD5 and dry remains were also identified. Sanitary-microbiological studies were performed using the following methods: mesophilic aerobes and facultative anaerobes: МУК, 2.1.4 1184-03, common coliform bacteria − МУК 4.2. 1018-01 and Escherichia coli − 18963-73. Based on the experimental data, it was found that the spring water that being researched is low-mineralized, which is why their reaction is almost neutral. The above-mentioned ionic content in the studied spring waters is within the norm, and in some samples (mesophilic aerobes and facultative anaerobes, common coliform bacteria) are established that microbiological, such a marginal concentration of pollution that is harmless for human health and its use in drinking and agricultural terms is within the norm.