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Keragaman genetik mimi (Carcinoscorpius rotundicauda dan Tachypleus gigas) di perairan Demak, Madura dan Balikpapan berdasarkan penanda Random Amplified Polymorphic DNA

Naila Khuril Aini, Ali Mashar, Hawis H. Madduppa, Yusli Wardiatno
Abstract: Horseshoe crab is an exotic and protected marine organism in Indonesia and is considered as the living fossil animal in the world. IUCN conservation status of Carcinoscorpius rotundicauda and Tachypleus gigas is still Data Deficient, and in Indonesia research on genetic population is lacking, if any. This study aims to reveal genetic diversity of C. rotundicauda and T. gigas populations in Demak, Madura, and Balikpapan coastal waters. This research was conducted by using molecular analysis with genetic markers Random Amplified Polymorphic DNA (RAPD). The primers used in this study were OPB 06, OPG 10, and OPX 03. The results showed that the highest polymorphism of C. rotundicauda was found in Demak (74.6667%) and heterozygosity was 0.2669. Furthermore, T. gigas had the highest polymorphism in Madura (74.3590%) and heterozygosity was 0.25551. Based on pairwise comparison tests, populations of C. rotundicauda and T. gigas in Demak, Madura, and Balikpapan were significantly different (p
Keywords: adaptation / diversity / polymorphism / Madura / Horseshoe Crabs / Gigas / Balikpapan / Rotundicauda / Demak

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