Electrochemical detection of aflatoxin B1: an aptasensor prepared using graphene oxide and gold nanowires
- 30 October 2019
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Analytical Methods
- Vol. 11 (47), 6033-6042
- https://doi.org/10.1039/c9ay01673b
Abstract
An electrochemical aptasensor was developed for the detection of aflatoxin B1 using a nanocomposite of graphene oxide and gold nanowires. The aptasensor mechanism was based on the difference in the differential pulse voltammetry peak current upon detachment of the aptamer from the electrode surface when the toxin is present. The working electrode modifications were confirmed using field emission scanning electron microscopy, energy-dispersive X-ray spectroscopy, and electrochemical methods cyclic voltammetry and electrochemical impedance spectroscopy. The limit of detection was 1.4 pM and the linear range was 5.0–750.0 pM with good reproducibility of the results. The specificity test of the aptasensor successfully confirmed that the aptasensor is specific towards aflatoxin B1 compared to a blank solution and non-specific molecules. A simulated real sample environment tested in pistachio samples alongside experiments and comparison with the HPLC method confirmed the aptasensor's capability to work in future real food assays.Keywords
This publication has 43 references indexed in Scilit:
- A novel electrochemical immunosensor for highly sensitive detection of aflatoxin B1 in corn using single-walled carbon nanotubes/chitosanFood Chemistry, 2016
- Electrochemical piezoelectric reusable immunosensor for aflatoxin B1 detectionBiochemical Engineering Journal, 2015
- A novel ultrasensitive phosphate amperometric nanobiosensor based on the integration of pyruvate oxidase with highly ordered gold nanowires arrayBiosensors and Bioelectronics, 2015
- Gold nanowires and nanotubes for high sensitivity detection of pathogen DNASensors and Actuators B: Chemical, 2015
- Highly sensitive electrochemical impedance spectroscopy immunosensor for the detection of AFB1 in olive oilFood Chemistry, 2015
- A novel electrochemical piezoelectric label free immunosensor for aflatoxin B1 detection in groundnutFood Control, 2014
- Methods for Detection of Aflatoxins in Agricultural Food CropsJournal of Applied Chemistry, 2014
- A label-free electrochemical impedance immunosensor for the sensitive detection of aflatoxin B1Analytical Methods, 2014
- Electrochemical Aptasensor Based on Polycarboxylic Macrocycle Modified with Neutral Red for Aflatoxin B1 DetectionElectroanalysis, 2014
- Introduction of hematoxylin as an electroactive label for DNA biosensors and its employment in detection of target DNA sequence and single-base mismatch in human papilloma virus corresponding to oligonucleotideBiosensors and Bioelectronics, 2011