Laboratory-acquired infections (LAIs) are defined as infections acquired through laboratory or laboratory-related activities. Whether the infected host remains asymptomatic or becomes symptomatic with overt illness depends on many unpredictable factors. A variety of microorganisms, viz. bacteria, viruses, rickettsiae, fungi and parasites cause LAIs. These infections are a hazard in personnel engaged in clinical research laboratories. An intensive search of literature through several search engines revealed that dimorphic fungi, viz. Blastomyces dermatitidis, Coccidioides immitis, and Histoplasma capsulatum are responsible for the maximum number of laboratory-acquired (LA) mycoses. Coccidioidomycosis caused by C. immiitis and dermatophytosis caused by Trichophyton mentagrophytes are the commonest laboratory acquired (LA) fungal infections. The aim of this study is to give an update of the present state of our knowledge on LA fungal infections and suggest preventive measures.