Kinesin-5 Eg5 mediates centrosome separation to control spindle assembly in spermatocytes
- 18 April 2022
- journal article
- research article
- Published by Springer Science and Business Media LLC in Chromosoma
- Vol. 131 (1-2), 87-105
- https://doi.org/10.1007/s00412-022-00772-5
Abstract
Timely and accurate centrosome separation is critical for bipolar spindle organization and faithful chromosome segregation during cell division. Kinesin-5 Eg5 is essential for centrosome separation and spindle organization in somatic cells; however, the detailed functions and mechanisms of Eg5 in spermatocytes remain unclear. In this study, we show that Eg5 proteins are located at spindle microtubules and centrosomes in spermatocytes both in vivo and in vitro. We reveal that the spermatocytes are arrested at metaphase I in seminiferous tubules after Eg5 inhibition. Eg5 ablation results in cell cycle arrest, the formation of monopolar spindle, and chromosome misalignment in cultured GC-2 spd cells. Importantly, we find that the long-term inhibition of Eg5 results in an increased number of centrosomes and chromosomal instability in spermatocytes. Our findings indicate that Eg5 mediates centrosome separation to control spindle assembly and chromosome alignment in spermatocytes, which finally contribute to chromosome stability and faithful cell division of the spermatocytes.Keywords
Funding Information
- National Natural Science Foundation of China (82001608)
- Natural Science Foundation of Fujian Province, China (2019J05071)
- Fujian Provincial Health Technology Project (2018-1-69)
- Fujian Provincial Health Technology Project (2020QNB009)
- Startup Fund for scientific research, Fujian Medical University (2017XQ1001)
- Fujian Medical University high level talents scientific research start-up funding project (XRCZX2017025)
- Research project of online education and teaching of Chinese medicine graduate students (B-YXC20200202-06)
This publication has 85 references indexed in Scilit:
- Nuclear envelope-associated dynein drives prophase centrosome separation and enables Eg5-independent bipolar spindle formationThe EMBO Journal, 2012
- Mechanisms of Centrosome Separation and Bipolar Spindle AssemblyDevelopmental Cell, 2010
- Mitotic functions of kinesin-5Seminars in Cell & Developmental Biology, 2010
- Dynein Antagonizes Eg5 by Crosslinking and Sliding Antiparallel MicrotubulesCurrent Biology, 2009
- The Role of Hklp2 in the Stabilization and Maintenance of Spindle BipolarityCurrent Biology, 2009
- Kif15 Cooperates with Eg5 to Promote Bipolar Spindle AssemblyCurrent Biology, 2009
- Chromosome Congression by Kinesin-5 Motor-Mediated Disassembly of Longer Kinetochore MicrotubulesCell, 2008
- Kinesin-5 acts as a brake in anaphase spindle elongationCurrent Biology, 2007
- The kinesin related motor protein, Eg5, is essential for maintenance of pre-implantation embryogenesisBiochemical and Biophysical Research Communications, 2007
- Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivoCell, 1995