Genetic Structure Analysis of Three Wild Populations of Coilia nasus in Liaoning Province Based on Mitochondrial D-loop Sequences

Abstract

The genetic structure of Coilia nasus was studied based on the sequences analysis of mitochondrial D-loop in 75 individuals from Liao River (n = 20), Dayang River (n = 27) and Yalu River (n = 28), Liaoning province. In 1130~1171 bp of D-loop sequences, there were 94 polymorphic sites and 60 haplotypes. The haplotype and nucleotide diversities were 0.992 ± 0.004 and 0.010 ± 0.000, respectively. AMOVA revealed 98.27% of the total variation was resulted from intra-population differentiation, there was little genetic differentiation among populations. Both NJ tree and MJ network of 60 haplotypes indicated there was no geographic structure among populations. Three populations of C. nasus should be considered as a genetic management unit. The findings may be very useful to protect this species stock.