Fatty acid profile, antioxidant and antibacterial effect of the ethyl acatate extract of cleistopholis patens
Abstract: Cleistopholis patens is a tropical plant that is used in the treatment of many bacterial and fungal infections. In this study, the antibacterial action against some human pathogens and the fatty acid profile of the plant were investigated. The plant was found to be active against Salmonella typhi Streptococcus pyogenes, Staphylococcus aureus while Shigella dysenteriae was resistant. The comparative antibiotic test revealed that only Staphylococcus aureus was sensitive to chloramphenicol and gentamycin. The purified extract showed lesser activity than the crude extract. Phytochemical components include; glycosides, steroids, Phenol, tanins and saponins. Anticonstituents include tanin (2.32mg/g), phenol (2.50 mg/g), phytate (15.65 mg/g), oxalate (6.57 mg/g), saponin (9.71 mg/g) and flavonoids (6.49 mg/g). The GCMS profile of the ethyl acetate extract of the plant revealed 23 fatty acid including 9-Hexadecanoic acid, (25.11%), 6 octadecanoic acid (21.98%), n Hexadecanoic (4.62%), Cyclopentaneundecanoic acid, methyl ester (2.05%). Heptacosanoic acid, methyl ester (2.05%), Decanoic acid, methyl ester (1.74%), Oleic acid (4.38%), 16-Octadecenoic acid, methyl ester (3.55%) amongst others.
Keywords: ester / methyl / tanins / aureus / Hexadecanoic / patens / Cleistopholis / Phenol / saponin
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