The article presents the phenomenon of forming the culture of future teachers’ safe use of information environment through the selection of emotional and motivational content. The emotional and motivational contents enabling the formation of the culture of a safe use of the information environment should be aimed at taking into account the personality of the future teacher, the tendencies and state of the modern information technologies, students’ interests and needs. Modern realities point to a gap in educational materials which do not keep step with the rapid development of information and communication technologies, which decreases future teachers’ motivation to form the information culture and the culture of safe use of the information environment in particular. The purpose of the article is to substantiate the need to select emotional and motivational content to form the culture of future teachers’ safe use of information environment and to highlight the positive impact of the emotional and motivational contents on the process. The objectives of the study were as follows: theoretical analysis of the scientific literature regarding the peculiarities of the content of emotional and motivational directions in the process of forming the culture of the future teachers’ safe use of information environment; clarification of priority content-centred topics aimed at developing the culture of the future teachers’ safe use of the information environment. In view of the specification of the tasks, in the research there have been used theoretical analysis of the scientific and pedagogical literature which made it possible to find out the modern approaches of researchers to the formation of the culture of the future teachers’ safe use of the information environment through the selection of emotional and motivational content. Some methods of empirical research have been used, including questionnaires, in order to clarify future teachers’ wishes and interests regarding the filling of the thematic content of the safe use of the information environment. The results of the study allowed revealing the topics that were of interested for the future teachers in terms of the safe use of the information environment: the efficiency and safety of navigation in the information environment, the impact of the irrational use of the information environment on physical and psychological health and ways to overcome it; information hygiene. Keywords: culture, safe use of the information environment, emotions, motives.