Lateral Flow Immunoassay Coupled with Copper Enhancement for Rapid and Sensitive SARS-CoV-2 Nucleocapsid Protein Detection
Open Access
- 29 December 2021
- journal article
- research article
- Published by MDPI AG in Biosensors
- Vol. 12 (1), 13
- https://doi.org/10.3390/bios12010013
Abstract
The coronavirus disease 2019 (COVID-19) pandemic caused by severe acute respiratory coronavirus 2 (SARS-CoV-2) is still raging all over the world. Hence, the rapid and sensitive screening of the suspected population is in high demand. The nucleocapsid protein (NP) of SARS-CoV-2 has been selected as an ideal marker for viral antigen detection. This study describes a lateral flow immunoassay (LFIA) based on colloidal gold nanoparticles for rapid NP antigen detection, in which sensitivity was improved through copper deposition-induced signal amplification. The detection sensitivity of the developed LFIA for NP antigen detection (using certified reference materials) under the optimized parameters was 0.01 μg/mL and was promoted by three orders of magnitude to 10 pg/mL after copper deposition signal amplification. The LFIA coupled with the copper enhancement technique has many merits such as low cost, high efficiency, and high sensitivity. It provides an effective approach to the rapid screening, diagnosis, and monitoring of the suspected population in the COVID-19 outbreak.Keywords
Funding Information
- the Central Public-interest Scientific Institution Basal Research Fund (AKYZD2111, 20K10ESPCT)
This publication has 25 references indexed in Scilit:
- Rapid Detection of COVID-19 Causative Virus (SARS-CoV-2) in Human Nasopharyngeal Swab Specimens Using Field-Effect Transistor-Based BiosensorACS Nano, 2020
- Labeling Antibodies Using Colloidal GoldCold Spring Harbor Protocols, 2020
- A new coronavirus associated with human respiratory disease in ChinaNature, 2020
- Copper deposition-induced efficient signal amplification for ultrasensitive lateral flow immunoassaySensors and Actuators B: Chemical, 2019
- Origin and evolution of pathogenic coronavirusesNature Reviews Microbiology, 2018
- A concentration−dependent multicolor conversion strategy for ultrasensitive colorimetric immunoassay with the naked eyeAnalytica Chimica Acta, 2017
- An ultrasensitive colorimetric strategy for protein O-GlcNAcylation detection via copper deposition-enabled nonenzymatic signal amplificationRSC Advances, 2016
- Novel Strategies To Enhance Lateral Flow Immunoassay Sensitivity for Detecting Foodborne PathogensJournal of Agricultural and Food Chemistry, 2015
- Monoclonal Antibody-Based Antigen Capture Enzyme-Linked Immunosorbent Assay Reveals High Sensitivity of the Nucleocapsid Protein in Acute-Phase Sera of Severe Acute Respiratory Syndrome PatientsClinical and Vaccine Immunology, 2005
- Nucleocapsid Protein as Early Diagnostic Marker for SARSEmerging Infectious Diseases, 2004