An Enzyme-Induced Novel Biosensor for the Sensitive Electrochemical Determination of Isoniazid
Open Access
- 5 June 2017
- journal article
- research article
- Published by MDPI AG in Biosensors
- Vol. 7 (2), 21
- https://doi.org/10.3390/bios7020021
Abstract
In this present work, a glassy carbon electrode (GCE) was modified primarily with multiwalled carbon nanotubes (MWCNTs) and a composite of MWCNTs and titanium oxide nanoparticles (TiO2NPs). The enzyme horseradish peroxidase (HRP) was immobilized to enhance the sensing ability of GCE. The proposed biosensor was used for the sensitive determination of isoniazid (INZ) in various pharmaceutical samples. The electrochemical behaviour of the developed MWCNT-TiO2NPs-HRP-GCE biosensor was studied by using cyclic voltammetry (CV) and differential pulse voltammetric (DPV) techniques. Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), thermogravimetry (TGA) and transmission electron microscopy (TEM) techniques were used to characterize the developed sensor. Phosphate buffer solution (PBS) with pH 7 was used as supporting electrolyte in the present investigation. The cyclic voltammetric results revealed that the increment of anodic peak currents for the enzyme-induced sensor was almost 8-fold greater than that of a bare GCE. The DPV technique exhibited good limit of detection and limit of quantification values, viz., 0.0335 μM and 0.1118 μM, respectively. Moreover, the developed sensor showed long-lasting stability and repeatability without any interferents. This strongly indicates that the fabricated sensor shows outstanding electrochemical performance towards INZ, with excellent selectivity and sensitivity. The developed sensor was successfully applied to pharmaceutical samples and gave good percentages of recoveries.Keywords
This publication has 35 references indexed in Scilit:
- Pt-TiO2/MWCNTs Hybrid Composites for Monitoring Low Hydrogen Concentrations in AirSensors, 2012
- Efficient electrocatalytic oxidation and selective determination of isoniazid by Fe(tmphen) 3 2+ -exchanged Nafion®-modified electrodeJournal of Solid State Electrochemistry, 2012
- Structural basis for pregnenolone biosynthesis by the mitochondrial monooxygenase systemProceedings of the National Academy of Sciences of the United States of America, 2011
- Determination of isoniazid in human urine using screen-printed carbon electrode modified with poly-l-histidineBioelectrochemistry, 2010
- Simultaneous voltammetric determination of ascorbic acid, acetaminophen and isoniazid using thionine immobilized multi-walled carbon nanotube modified carbon paste electrodeElectrochimica Acta, 2010
- Direct Electrochemistry of Horseradish Peroxidase-Gold Nanoparticles ConjugateSensors, 2009
- Biowaiver Monographs for Immediate Release Solid Oral Dosage Forms: Isoniazid**A project of the International Pharmaceutical Federation FIP, Groupe BCS, www.fip.org/bcs.Journal of Pharmaceutical Sciences, 2007
- Multi-walled carbon nanotube paste electrode for selective voltammetric detection of isoniazidMicrochimica Acta, 2006
- Estimation of antitubercular drugs combination in pharmaceutical formulations using multivariate calibrationAnalytica Chimica Acta, 2005
- Colorimetric and titrimetric assay of isoniazidJournal of Pharmaceutical and Biomedical Analysis, 1992