Activity Of Amomum Tasao-Ko Fruits Essential Oil Against Methicillin-Resistant Staphylococcus Aureus In Vivo

Abstract

Methicillin-Resistant Staphylococcus Aureus (MRSA) is one of the most serious health concerns bacterial owing to its incidence and the severity of the associated infections. It is necessary to find new chemical compounds with anti-MRSA activity. A model of MRSA infection was prepared by intraperitoneal injection of MRSA. A. tasao-ko essential oil was used for pretreatment and treatment of MRSA-infected animals and the 50% effective dose (ED50) was calculated according to improved Karber’s method respectively. The levels of serum interleukin (IL)-1β, IL-6, Tumor Necrosis Factor alpha (TNF-α), Malondialdehyde (MDA), Glutathione Peroxidase (GSH-Px) and Hydroxyl Radical (·OH) were measured using dedicated assay kits. The statistical analysis of inflammatory factors and oxidative stress factors was tested using one-way ANOVA computed by SPSS software. The ED50 values for the prevention and treatment of MRSA infection in A. tasao-ko essential oil were 0.15 g/kg and 0.18 g/kg, respectively. A. tasao-ko essential oil significantly reduced the inflammatory factors (IL-1β, IL-6, TNF-α), oxidative stress factors (MDA, ·OH) and increased the GSH-Px content in mice infected with MRSA. In the high-dose group, 7 days after treatment, the cytokine contents have no difference with control group (P > 0.05). A. tasao-ko essential oil exhibited anti-MRSA effect in vivo and can also ameliorate the abnormal changes in inflammatory factors and oxidative stress that arise from MRSA.