Multi-Omics Analysis to Generate Hypotheses for Mild Health Problems in Monkeys
Open Access
- 13 October 2021
- journal article
- research article
- Published by MDPI AG in Metabolites
- Vol. 11 (10), 701
- https://doi.org/10.3390/metabo11100701
Abstract
Certain symptoms associated with mild sickness and lethargy have not been categorized as definitive diseases. Confirming such symptoms in captive monkeys (Macaca fascicularis, known as cynomolgus monkeys) can be difficult; however, it is possible to observe and analyze their feces. In this study, we investigated the relationship between stool state and various omics data by considering objective and quantitative values of stool water content as a phenotype for analysis. By examining the food intake of the monkeys and assessing their stool, urine, and plasma, we attempted to obtain a comprehensive understanding of the health status of individual monkeys and correlate it with the stool condition. Our metabolomics data strongly suggested that many lipid-related metabolites were correlated with the stool water content. The lipidomic analysis revealed the involvement of saturated and oxidized fatty acids, metallomics revealed the contribution of selenium (a bio-essential trace element), and intestinal microbiota analysis revealed the association of several bacterial species with the stool water content. Based on our results, we hypothesize that the redox imbalance causes minor health problems. However, it is not possible to make a definite conclusion using multi-omics alone, and other hypotheses could be proposed.Funding Information
- Japan Microbiome Consortium (JMBC2018 collaboration)
This publication has 60 references indexed in Scilit:
- Predicting Network Activity from High Throughput MetabolomicsPLoS Computational Biology, 2013
- Global Prevalence of and Risk Factors for Irritable Bowel Syndrome: A Meta-analysisClinical Gastroenterology and Hepatology, 2012
- Phosphatidylethanol in blood (B‐PEth): A marker for alcohol use and abuseDrug Testing and Analysis, 2011
- Recent Progress on Phospholipases: Different Sources, Assay Methods, Industrial Potential and PathogenicityApplied Biochemistry and Biotechnology, 2011
- Lipid map of the mammalian cellJournal of Cell Science, 2011
- Effect of plasma triglyceride metabolism on lipid storage in adipose tissue: Studies using genetically engineered mouse modelsBiochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 2009
- Bacterial lipid composition and the antimicrobial efficacy of cationic steroid compounds (Ceragenins)Biochimica et Biophysica Acta (BBA) - Biomembranes, 2007
- Naïve Bayesian Classifier for Rapid Assignment of rRNA Sequences into the New Bacterial TaxonomyApplied and Environmental Microbiology, 2007
- Oxidative stress and redox regulation of phospholipase D in myocardial diseaseFree Radical Biology & Medicine, 2006
- A multiplex quantitation method for eicosanoids and platelet-activating factor using column-switching reversed-phase liquid chromatography–tandem mass spectrometryAnalytical Biochemistry, 2005