In vitro and in vivo assays for osteoclast apoptosis
Open Access
- 1 January 2005
- journal article
- Published by Springer Science and Business Media LLC in Biological Procedures Online
- Vol. 7 (1), 48-59
- https://doi.org/10.1251/bpo105
Abstract
Mature osteoclasts, multinucleated giant cells responsible for bone resorption, are terminally differentiated cells with a short life span. Recently, we have demonstrated that osteoclast apoptosis is regulated by ERK activity and Bcl-2 family member Bim. In this paper, we summarize the methods we used to study osteoclast apoptosis in vitro and in vivo. Using adenovirus and retrovirus vectors, we were able to introduce foreign genes into osteoclasts and examine their effects on osteoclast survival in vitro. In addition, we established the modified methods for in situ hybridization and BrdU labeling of bone sections from mice to study osteoclast survival in vivo. The detailed methods described here could be useful for studying the biological process in bone.Keywords
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