Correlations between microbiota and metabolites after faecal microbiota transfer in irritable bowel syndrome
Open Access
- 24 February 2021
- journal article
- research article
- Published by Brill in Beneficial Microbes
- Vol. 12 (1), 17-30
- https://doi.org/10.3920/bm2020.0010
Abstract
Faecal microbiota transfer (FMT) consists of the infusion of donor faecal material into the intestine of a patient with the aim to restore a disturbed gut microbiota. In this study, it was investigated whether FMT has an effect on faecal microbial composition, its functional capacity, faecal metabolite profiles and their interactions in 16 irritable bowel syndrome (IBS) patients. Faecal samples from eight different time points before and until six months after allogenic FMT (faecal material from a healthy donor) as well as autologous FMT (own faecal material) were analysed by 16S RNA gene amplicon sequencing and gas chromatography coupled to mass spectrometry (GS-MS). The results showed that the allogenic FMT resulted in alterations in the microbial composition that were detectable up to six months, whereas after autologous FMT this was not the case. Similar results were found for the functional profiles, which were predicted from the phylogenetic sequencing data. While both allogenic FMT as well as autologous FMT did not have an effect on the faecal metabolites measured in this study, correlations between the microbial composition and the metabolites showed that the microbe-metabolite interactions seemed to be disrupted after allogenic FMT compared to autologous FMT. This shows that FMT can lead to altered interactions between the gut microbiota and its metabolites in IBS patients. Further research should investigate if and how this affects efficacy of FMT treatments.Keywords
This publication has 50 references indexed in Scilit:
- Drug-Resistant E. coli Bacteremia Transmitted by Fecal Microbiota TransplantThe New England Journal of Medicine, 2019
- Allogenic Faecal Microbiota Transfer Induces Immune-Related Gene Sets in the Colon Mucosa of Patients with Irritable Bowel SyndromeBiomolecules, 2019
- The Effect of Allogenic Versus Autologous Fecal Microbiota Transfer on Symptoms, Visceral Perception and Fecal and Mucosal Microbiota in Irritable Bowel Syndrome: A Randomized Controlled StudyClinical and Translational Gastroenterology, 2019
- Effect of Fecal Microbiota Transplantation on 8-Week Remission in Patients With Ulcerative ColitisJAMA, 2019
- Faecal microbiota transplantation alters gut microbiota in patients with irritable bowel syndrome: results from a randomised, double-blind placebo-controlled studyGut, 2018
- Regulation of immune cell function by short‐chain fatty acidsClinical & Translational Immunology, 2016
- Instability of the faecal microbiota in diarrhoea-predominant irritable bowel syndromeFEMS Microbiology Ecology, 2013
- Fame and future of faecal transplantations – developing next‐generation therapies with synthetic microbiomesMicrobial Biotechnology, 2013
- Amino acid metabolism in intestinal bacteria: links between gut ecology and host healthFrontiers in Bioscience-Landmark, 2011
- Biplots of Compositional DataJournal of the Royal Statistical Society Series C: Applied Statistics, 2002