Cs-131 as an experimental tool for the investigation and quantification of the radiotoxicity of intracellular Auger decays in vitro
Open Access
- 15 July 2020
- journal article
- research article
- Published by Taylor & Francis Ltd in International Journal of Radiation Biology
- Vol. 99 (1), 39-52
- https://doi.org/10.1080/09553002.2020.1787541
Abstract
Purpose: In this work, we set out to provide an experimental setup, using Cs-131, with associated dosimetry for studying relative biological effectiveness (RBE) of Auger emitters. Material & Methods: Cs-131 decays by 100% electron capture producing K- (9%) and L- (80%) Auger electrons with mean energies of 26 keV and 3.5 keV, respectively, plus ≈ 9.4 very low energy electrons (+/K+-ATPase. By this uptake mechanism and the alkali chemistry of Cs+, we argue for its intracellular homogeneous distribution. Cs-131 was added to the cell culture medium of HeLa and V79 Cells. The bio-kinetics of Cs-131 (uptake, release, intracellular distribution) was examined by measuring its intracellular activity concentration over time. Taking advantage of the 100% confluent cellular monolayer, we developed a new and robust dosimetry that is entrusted to a quantity called SC-value. Results: The SC-values evaluated in the cell nucleus are almost independent of the nuclear size and geometry. We obtained dose-rate controlled RBE-values for intracellular Cs-131 decay. Using the γH2AX assay, the RBE was 1 for HeLa cells. Using the clonogenic cell survival, it was 3.9 for HeLa cells and 3.2 for V79 cells. Conclusion: This experimental setup and dosimetry provides reliable RBE-values for Auger emitters in various cell lines.Keywords
This publication has 43 references indexed in Scilit:
- Na+, K+-ATPase: Ubiquitous Multifunctional Transmembrane Protein and its Relevance to Various Pathophysiological ConditionsJournal of Clinical Medicine Research, 2010
- γH2AX and cancerNature Reviews Cancer, 2008
- The Amazing World of Auger ElectronsInternational Journal of Radiation Biology, 2004
- Microchemical Element Imaging of Yeast and Human Cells Using Synchrotron X-ray Microprobe with Kirkpatrick−Baez OpticsAnalytical Chemistry, 2003
- A review of dsb induction data for varying quality radiationsInternational Journal of Radiation Biology, 1998
- Transport kinetics, cation inhibition and intracellular location of accumulated caesium in the green microalga Chlorella salinaJournal of General Microbiology, 1993
- Caesium accumulation and interactions with other monovalent cations in the cyanobacterium Synechocystis PCC 6803Microbiology, 1991
- The distribution of intracellular alkali metals in Reference ManPhysics in Medicine & Biology, 1987
- Conduction and selectivity in potassium channelsThe Journal of Membrane Biology, 1983
- Tilden Lecture. The biochemistry of sodium, potassium, magnesium, and calciumQuarterly Reviews, Chemical Society, 1970