3,3′-Diindolylmethane (DIM): A Potential Therapeutic Agent against Cariogenic Streptococcus mutans Biofilm
Open Access
- 6 June 2023
- journal article
- research article
- Published by MDPI AG in Antibiotics
- Vol. 12 (6), 1017
- https://doi.org/10.3390/antibiotics12061017
Abstract
Indole, a metabolite of the amino acid tryptophan, has been proven to act as a signal molecule in bacteria, acting in different aspects of biofilm formation. The oral biofilm is a type of biofilm that has consequences for human health. It is a complex, three-dimensional structure that develops on the surface of teeth via the attachment of primary microbial colonizers. Many oral infections are caused by an imbalance occurring in the microorganisms naturally found in oral biofilms and are considered major public health concerns. In this study, we test the effect of a natural bis-indole, 3,3′-Diindolylmethane (DIM), in mitigating the pathogenicity of the oral biofilm inhabiting bacterium Streptococcus mutans, a bacterium that is considered to be a principal etiological agent in dental caries. Our study found that DIM was able to attenuate S. mutans biofilm formation by 92%. Additionally, treatment with DIM lowered extracellular polymeric substance (EPS) production and decreased its durability significantly under acidic conditions. Therefore, the anti-biofilm and anti-virulence properties of DIM against S. mutans bacteria in an “oral setting” provides evidence for its usefulness in reducing biofilm formation and potentially for caries attenuation.Keywords
Funding Information
- International Research and Development Program of Sichuan (2019YFH0113)
- SMART innovation (ING-000398)
This publication has 49 references indexed in Scilit:
- A Family of Indoles Regulate Virulence and Shiga Toxin Production in Pathogenic E. coliPLOS ONE, 2013
- Bursting the bubble on bacterial biofilms: a flow cell methodologyBiofouling, 2012
- Signaling-mediated bacterial persister formationNature Chemical Biology, 2012
- The Tea Catechin Epigallocatechin Gallate Suppresses Cariogenic Virulence Factors of Streptococcus mutansAntimicrobial Agents and Chemotherapy, 2011
- Indole Affects Biofilm Formation in BacteriaIndian Journal of Microbiology, 2010
- Protocols to Study the Physiology of Oral BiofilmsMethods in molecular biology (Clifton, N.J.), 2010
- Acid tolerance mechanisms utilized by Streptococcus mutansFuture Microbiology, 2010
- A model of efficiency: stress tolerance by Streptococcus mutansMicrobiology, 2008
- Salivary Glucosyltransferase B as a Possible Marker for Caries ActivityCaries Research, 2007
- Indole is an inter-species biofilm signal mediated by SdiABMC Microbiology, 2007