Laser-Induced MoOx/Sulfur-Doped Graphene Hybrid Frameworks as Efficient Antibacterial Agents

Abstract

Rational design and scalable construction of antibacterial mediators based on unique graphene architectures with highly efficient antibacterial ability and significant biocompatibility are challenging. Herein, sulfur-doped graphene skeletons uniformly decorated with metal oxide nanoparticles were designed and constructed via one-step laser-induced microexplosive techniques and demonstrated for the first time as highly efficient antibacterial agents. The optical density and flat colony counting methods demonstrated that the as-designed laser-induced MoO_x/sulfur-doped graphene hybrids exhibited exceptional activity inhibition of Escherichia coli and Staphylococcus aureus. Moreover, the bacteria were treated with an impressive laser-induced MoO_x/sulfur-doped graphene colloidal solution of concentration as low as 1 mg/mL for 4 h, leading to an excellent viability loss of 85% for the two bacteria. Cell toxicity experiments proved that the biological toxicity of laser-induced MoO_x/sulfur-doped graphene to pig sperm cells was negligible. The molecular dynamics calculations proposed that the intrinsic interaction with N-acetylglucosamine at the cell wall and the high-efficiency synergistic effect of sulfur-doped graphene and MoO_x played the key role in inhibiting the viability of bacteria. This work provides new insights for a novel structure design and opens up a potential route to construct antibacterial agents with high efficiency for clinical application.