Pathogenic Assessment of SfMNPV-Based Biopesticide on Spodoptera frugiperda (Lepidoptera: Noctuidae) Developing on Transgenic Soybean Expressing Cry1Ac Insecticidal Protein
- 6 September 2021
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Economic Entomology
- Vol. 114 (6), 2264-2270
- https://doi.org/10.1093/jee/toab170
Abstract
Pathogenic assessment of a baculovirus-based biopesticide containing Spodoptera frugiperda multiple nucleopolyhedrovirus (SfMNPV: Baculoviridae: Alphabaculovirus) infecting fall armyworm, Spodoptera frugiperda (J. E. Smith, 1797) (Lepidoptera: Noctuidae) is reported. In the bioassays, neonates were infected with different doses of SfMNPV applied on Cry1Ac Bt soybean and non-Bt soybean. Our findings indicated that S. frugiperda neonates did not survive at 10 d post infection or develop into adults on Bt and non-Bt soybean sprayed with the field recommended dose of SfMNPV. In contrast, a proportion of the infected neonates developed into adults when infected with lower doses of SfMNPV (50%, 25%, and 10% of field dose) in both Bt and non-Bt soybean. However, S. frugiperda neonates surviving infection at the lowest virus doses on both soybean varieties showed longer neonate-to-pupa and neonate-to-adult periods, lower larval and pupal weights, reduced fecundity, and increased population suppression. Nevertheless, more pronounced pathogenicity of SfMNPV infecting neonates of S. frugiperda were verified on larvae that developed on Bt soybean. These findings revealed that, beyond mortality, the biopesticide containing SfMNPV also causes significant sublethal pathogenic effects on neonates of S. frugiperda developing on Bt and non-Bt soybean and suggested an additive effect among SfMNPV and Cry1Ac insecticidal protein expressed in Bt soybean.Keywords
Funding Information
- Rio Grande do Sul Research Support Foundation (9/2551-0001754-5)
- National Council of Technological and Scientific Development (305464/2020-5, 304018/2019-8)
This publication has 33 references indexed in Scilit:
- Investigating the Molecular Mechanisms of Organophosphate and Pyrethroid Resistance in the Fall Armyworm Spodoptera frugiperdaPLOS ONE, 2013
- Intra- and Intergenerational Persistence of an Insect Nucleopolyhedrovirus: Adverse Effects of Sublethal Disease on Host Development, Reproduction, and Susceptibility to SuperinfectionApplied and Environmental Microbiology, 2011
- Lepidopteran larva consumption of soybean foliage: basis for developing multiple‐species economic thresholds for pest management decisionsPest Management Science, 2010
- Sub-lethal effects ofSpltMNPV infection on developmental stages ofSpodoptera litura(Lepidoptera: Noctuidae)Biocontrol Science and Technology, 2008
- Herança da resistência de Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) a lambda-cialotrinaNeotropical Entomology, 2001
- Influence of Larval Age on the Lethal and Sublethal Effects of the Nucleopolyhedrovirus ofTrichoplusia niin the Cabbage LooperBiological Control, 1998
- Sublethal Effects of Baculovirus in the Cabbage Moth, Mamestra brassicaeBiological Control, 1995
- The Effects of a Sublethal Baculovirus Infection in the Indian Meal Moth, Plodia interpunctellaJournal of Animal Ecology, 1994
- The effect of gypsy moth metamorphosis on the development of nuclear polyhedrosis virus infectionJournal of Invertebrate Pathology, 1991
- Pathogenicity of AcMNPV for larvae of Boarmia selenaria, Heliothis armigera, Heliothis peltigera, Spodoptera littoralis, and Ephestia cautellaJournal of Invertebrate Pathology, 1989