Solid-State Fermentation Production of Chitosanase by Streptomyces with Waste Mycelia of Aspergillus niger
Open Access
- 1 January 2021
- journal article
- research article
- Published by Scientific Research Publishing, Inc. in Advances in Enzyme Research
- Vol. 09 (01), 10-18
- https://doi.org/10.4236/aer.2021.91002
Abstract
Solid-state fermentation was carried out using mycelium powder of Aspergillus niger as substrate for the production of chitosanase of Streptomyces. Results of the experiments indicated that the optimal medium consisted of wheat bran and mycelium powder of Aspergillus niger with initial moisture content of 60% - 70%. The enzyme activity reached 41.33 U per gram dry medium after cultured for 5 days at 28°C - 30°C and an initial pH 6.5. Chitosanase was detected on the second day of incubation and had maximal activity at 5 days and decreased gradually within a 1 month period. Solid-state fermentation is maybe an economic alternative in the production.Keywords
This publication has 29 references indexed in Scilit:
- Purification, characterization, and action mode of a chitosanase from Streptomyces roseolus induced by chitinCarbohydrate Research, 2012
- Chitooligosaccharides and N-acetyl-D-glucosamine stimulate peripheral blood mononuclear cell-mediated antitumor immune responsesMolecular Medicine Reports, 2012
- In vitro screening for anti-microbial activity of chitosans and chitooligosaccharides, aiming at potential uses in functional textilesJournal of Microbiology and Biotechnology, 2010
- Applications of biopolymers I: chitosanMonatshefte für Chemie / Chemical Monthly, 2009
- Inhibitory effects of chitooligosaccharides on tumor growth and metastasisFood and Chemical Toxicology, 2009
- Production of transglutaminase byStreptomycesisolates in solid-state fermentationLetters in Applied Microbiology, 2008
- Enzymatic production and biological activities of chitosan oligosaccharides (COS): A reviewCarbohydrate Polymers, 2005
- Amylase production in solid state fermentation by the thermophilic fungus Thermomyces lanuginosusJournal of Bioscience and Bioengineering, 2005
- Quantitative Determination of Chitosan and the Percentage of Free Amino GroupsAnalytical Biochemistry, 1993
- Chelating, film‐forming, and coagulating ability of the chitosan–glucan complex from Aspergillus niger industrial wastesBiotechnology & Bioengineering, 1980