Three-photon neuronal imaging in deep mouse brain
Top Cited Papers
- 7 August 2020
- journal article
- review article
- Published by Optica Publishing Group in Optica
- Vol. 7 (8), 947-960
- https://doi.org/10.1364/optica.395825
Abstract
Three-photon microscopy has been increasingly adopted for probing neural activities beyond the typical two-photon imaging depth. In this review, we outline the unique properties that differentiate three-photon microscopy from two-photon microscopy for in vivo imaging in biological samples, especially in the mouse brain. We present a systematic summary of the optimization of three-photon imaging parameters for neural imaging, based on their effects on calcium imaging quality and perturbation to brain tissues. Furthermore, we review the existing techniques for volumetric imag-ing and discuss their prospects in mesoscale three-photon imaging in deep tissue. (C) 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement p https://doi.org/10.1364/OPTICA.395825Keywords
Funding Information
- National Science Foundation (DBI-1707312)
- Cornell Neurotech Mong Fellowship
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