Increasing the Production of β-Glucan from Saccharomyces carlsbergensis RU01 by Using Tannic Acid
Open Access
- 31 March 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Applied Biochemistry and Biotechnology
- Vol. 193 (8), 2591-2601
- https://doi.org/10.1007/s12010-021-03553-5
Abstract
In this study, we increased β-glucan production from brewer’s yeast, Saccharomyces carlsbergensis RU01, by using tannic acid. High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone. The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid. The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w. Tannic acid enhanced the β-glucan content by up to 42.23%. β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture. Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control. In addition, β-glucans from brewer’s yeast can be used as prebiotic and functional foods for human health and in animal feed.Keywords
Funding Information
- National Research Council of Thailand (2560A11802129)
This publication has 35 references indexed in Scilit:
- Visualization of yeast cells by electron microscopyMicroscopy, 2012
- Chemistry, physico-chemistry and applications linked to biological activities of β-glucansNatural Product Reports, 2011
- Brewer's Saccharomyces yeast biomass: characteristics and potential applicationsTrends in Food Science & Technology, 2010
- Association of Beta-Glucan Endogenous Production with Increased Stress Tolerance of Intestinal LactobacilliApplied and Environmental Microbiology, 2010
- A new isolation method of β-d-glucans from spent yeast Saccharomyces cerevisiaeFood Hydrocolloids, 2008
- Yeast whole glucan particle (WGP) β-glucan in conjunction with antitumour monoclonal antibodies to treat cancerExpert Opinion on Biological Therapy, 2005
- Preparation of spent brewer's yeast β‐glucans for potential applications in the food industryInternational Journal of Food Science & Technology, 2003
- Dynamics of cell wall structure inSaccharomyces cerevisiaeFEMS Microbiology Reviews, 2002
- Sensitivity ofSaccharomyces cerevisiaeto tannic acid is due to iron deprivationCanadian Journal of Microbiology, 2001
- FTIR spectroscopic analysis ofSaccharomyces cerevisiaecell walls: study of an anomalous strain exhibiting a pink-colored cell phenotypeFEMS Microbiology Letters, 2001