Quality of corneal lamellar cuts quantified using atomic force microscopy
- 1 January 2013
- journal article
- Published by Ovid Technologies (Wolters Kluwer Health) in Journal of Cataract & Refractive Surgery
- Vol. 39 (1), 110-117
- https://doi.org/10.1016/j.jcrs.2012.07.040
Abstract
S were performed on 3 pairs of human cadaver eyes. The cornea was thinned to physiologic levels by placing the globe, cornea side down, in 25% dextran for 24 hours. The eyes were reinflated to normal pressures by injecting a balanced salt solution into the vitreous cavity. The eyes were placed in a holder, the epithelium was removed, and the eyes were cut with a Visumax femtosecond laser. The energy level was 180 nJ for the right eye and 340 nJ for the left eye of each pair. The cut depths were 200 μm, 300 μm, and 400 μm, with the cut depth maintained for both eyes of each pair. A 12.0 mm trephination was then performed. The anterior portion of the lamellar surface was placed in a balanced salt solution and imaged with AFM. As a control, the posterior surface was placed in 2% formalin and imaged with environmental scanning electron microscopy (SEM). Four quantitative parameters (root-mean-square deviation, average deviation, skewness, kurtosis) were calculated from the AFM images. Results From AFM, the 300 μm low-energy cuts were the smoothest. Similar results were seen qualitatively in the environmental SEM images. Conclusion Atomic force microscopy provided quantitative information on the quality of lamellar dissections made using a femtosecond laser, which is useful in optimizing patient outcomes in refractive and lamellar keratoplasty surgeries. Financial Disclosure No author has a financial or proprietary interest in any material or method mentioned....Keywords
Funding Information
- Carl Zeiss Meditec AG
- Florida Lions Eye Bank
- National Institutes of Health (P30-EY014801, F31-EY-21714-01)
- Research to Prevent Blindness
- National Science Foundation (MRI 0722372)
- James and Esther King Program, Florida Department of Health
This publication has 27 references indexed in Scilit:
- Conjunctival Lymphoma in a ChildCornea, 2011
- Sixty-Kilohertz Femtosecond Laser–Assisted Endothelial Keratoplasty: Clinical Results and Stromal Bed Quality EvaluationCornea, 2011
- The Applications of Atomic Force Microscopy to Vision ScienceInvestigative Ophthalmology & Visual Science, 2010
- Femtosecond Laser-Assisted Keratoplasty: Laboratory Studies in Eye Bank EyesCurrent Eye Research, 2009
- Atomic force microscopy analysis of normal and photoablated porcine corneasJournal of Biomechanics, 2006
- The Subfibrillar Arrangement of Corneal and Scleral Collagen Fibrils as Revealed by Scanning Electron and Atomic Force Microscopy.Archives of Histology and Cytology, 2000
- Atomic Force Microscopic Studies of Isolated Collagen Fibrils of the Bovine Cornea and Sclera.Archives of Histology and Cytology, 1997
- Atomic force microscopy of the cornea and scleraCurrent Eye Research, 1995
- Theory for the Atomic Force Microscopy of Deformable SurfacesPhysical Review Letters, 1989
- Atomic Force MicroscopePhysical Review Letters, 1986