N6-Methyladenosine Related Long Non-Coding RNAs and Immune Cell Infiltration in the Tumor Microenvironment of Gastric Cancer
Open Access
- 1 August 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in Biological Procedures Online
- Vol. 23 (1), 1-18
- https://doi.org/10.1186/s12575-021-00152-w
Abstract
To illustrate the influence of N6-methyladenosine long non-coding RNAs and immune cell infiltration in gastric cancer. We downloaded workflow-type data and clinical data from The Cancer Genome Atlas project. The relationship of lncRNA and m6A was identified. Kyoto Encyclopedia of Genes and Genomes gene expression enrichment analysis was performed. Lasso regression was utilized to construct a prognostic model. Survival analysis to explore the relationship between m6A lncRNA and clinical survival data. Differential analysis of the tumor microenvironment and immune correlation analysis to determine immune cell infiltration levels and their correlation with clinical prognosis. Co-expression analysis indicated that lncRNA expression was associated closely with m6A. m6A-lncRNAs were partially highly expressed in tumor tissue and could be used in a prognostic model to predict GC prognosis, independent of other clinical characteristics. “ADIPPOCYTOKINE SIGNALING PATHWAY” was most significantly enriched according to GSEA. ACBD3-AS1 was overexpressed in tumor tissue. Naïve B cell, Plasma cells, resting CD4 memory T cell were highly infiltrated tissues in cluster 2, while Macrophages M2, resting Mast cells, Monocytes, regulates T cells were lowly in cluster 1. All related scores were higher in cluster 2, indicating a lower purity of tumor cells and higher density of immune-related cells in the tumor microenvironment. m6A lncRNA is closely related to the occurrence and progression of GC. The corresponding prognostic model can be utilized to evaluate the prognosis of GC. m6A lncRNA and related immune cell infiltration in the tumor microenvironment can provide novel therapeutic targets for further research.Keywords
Funding Information
- National Natural Science Foundation of China (81860433, 81960436)
This publication has 33 references indexed in Scilit:
- N6-methyladenosine links RNA metabolism to cancer progressionCell Death & Disease, 2018
- N 6-methyladenosine alters RNA structure to regulate binding of a low-complexity proteinNucleic Acids Research, 2017
- METTL14 suppresses the metastatic potential of hepatocellular carcinoma by modulating N 6‐methyladenosine‐dependent primary MicroRNA processingJournal of Hepatology, 2016
- Post-transcriptional gene regulation by mRNA modificationsNature Reviews Molecular Cell Biology, 2016
- m6A RNA methylation promotes XIST-mediated transcriptional repressionNature, 2016
- Long Noncoding RNAs in Cancer PathwaysCancer Cell, 2016
- A Practical Guide to The Cancer Genome Atlas (TCGA)Published by Springer Science and Business Media LLC ,2016
- Tumor-associated macrophages of the M2 phenotype contribute to progression in gastric cancer with peritoneal disseminationGastric Cancer, 2015
- The emerging role of lncRNAs in cancerNature Medicine, 2015
- HNRNPA2B1 Is a Mediator of m6A-Dependent Nuclear RNA Processing EventsCell, 2015