Objective: To compare the content of lupanone and stigmasterol in the root, stem and leaf of Plan-tain plantain, and to provide a strong basis for the resource development and utilization of plantain. Methods: HPLC was used. Diamonsil-C18 (4.6 mm × 250 mm, 5 µm) column; Mobile phase: metha-nol-acetonitrile (50:50 by volume), isometric elution; Detection wavelength: 206 nm; Flow rate: 0.8 ml/min; Column temperature: 30˚C; Injection volume: 10 μL. SPSS 26.0 and SIMCA 11.5 were used to analyze the contents of lupanone and stigmasterol in plantain roots, stems and leaves by statisti-cal analysis, cluster analysis and principal component analysis. Results: The concentrations of lu-panone and stigmasterol had a good linear relationship with the peak areas in the range of 6.26~150.30 µg/ml and 11.26~270.20 µg/ml, respectively. The average recoveries were 95%~105%. The contents of lupanone in the roots and stems of 14 batches of Plantains were 94.46~917.23 µg/g and 180.82~1667.86 µg/g, and the contents of stigmasterol were 117.27~315.08 µg/g and 271.26~1186.74 µg/g. The results of statistical analysis showed that there were significant differences in the contents of lupanone and stigmasterol in different parts of M. japonica from the same plant (P < 0.05), but no significant differences were detected in M. japon-ica leaves. The results of principal component analysis and cluster analysis were consistent with those of statistical analysis. Conclusion: The results showed that the content of banana stem was higher than that of banana root. In order to make better use of drug resources, banana stem could be used as the source of lupanone and stigmasterol.